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An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon

Persimmon fruits accumulate a large amount of proanthocyanidins (PAs) during development. PAs cause a dry or puckering sensation due to its astringency. Pollination constant and non-astringent (PCNA) persimmon fruits can lose astringency during fruit ripening. However, little is known about the mech...

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Autores principales: Chen, Wenxing, Xiong, Yalou, Xu, Liqing, Zhang, Qinglin, Luo, Zhengrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356345/
https://www.ncbi.nlm.nih.gov/pubmed/28304376
http://dx.doi.org/10.1038/srep44671
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author Chen, Wenxing
Xiong, Yalou
Xu, Liqing
Zhang, Qinglin
Luo, Zhengrong
author_facet Chen, Wenxing
Xiong, Yalou
Xu, Liqing
Zhang, Qinglin
Luo, Zhengrong
author_sort Chen, Wenxing
collection PubMed
description Persimmon fruits accumulate a large amount of proanthocyanidins (PAs) during development. PAs cause a dry or puckering sensation due to its astringency. Pollination constant and non-astringent (PCNA) persimmon fruits can lose astringency during fruit ripening. However, little is known about the mechanism of natural de-astringency of Chinese PCNA (CPCNA). To gain insight into the molecular events of CPCNA natural de-astringency, we used mRNA-seq and iTRAQ-based quantitative proteomic analysis to measure changes in genes and proteins expression at two key stages of natural astringency removal (i.e. 10 and 20 weeks after bloom) and water-treated (i.e. 40 °C·12 h) de-astringency fruits. Our analyses show that the three predominantly process in CPCNA de-astringency: (1) water treatment strongly up-regulates glycolysis/acetaldehyde metabolism, (2) expression of genes/proteins involved in PA biosynthetic pathway was remarkably reduced in natural and water-treated de-astringency, (3) sugar metabolism and ethylene related pathway were quite abundant in natural de-astringency. We also found ethylene-related TFs were quite abundant in natural de-astringency, followed by WRKY and NAC transcription factors. These results provide an initial understanding of the predominantly biological processes underlying the natural de-astringency and “coagulation effect” in CPCNA.
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spelling pubmed-53563452017-03-22 An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon Chen, Wenxing Xiong, Yalou Xu, Liqing Zhang, Qinglin Luo, Zhengrong Sci Rep Article Persimmon fruits accumulate a large amount of proanthocyanidins (PAs) during development. PAs cause a dry or puckering sensation due to its astringency. Pollination constant and non-astringent (PCNA) persimmon fruits can lose astringency during fruit ripening. However, little is known about the mechanism of natural de-astringency of Chinese PCNA (CPCNA). To gain insight into the molecular events of CPCNA natural de-astringency, we used mRNA-seq and iTRAQ-based quantitative proteomic analysis to measure changes in genes and proteins expression at two key stages of natural astringency removal (i.e. 10 and 20 weeks after bloom) and water-treated (i.e. 40 °C·12 h) de-astringency fruits. Our analyses show that the three predominantly process in CPCNA de-astringency: (1) water treatment strongly up-regulates glycolysis/acetaldehyde metabolism, (2) expression of genes/proteins involved in PA biosynthetic pathway was remarkably reduced in natural and water-treated de-astringency, (3) sugar metabolism and ethylene related pathway were quite abundant in natural de-astringency. We also found ethylene-related TFs were quite abundant in natural de-astringency, followed by WRKY and NAC transcription factors. These results provide an initial understanding of the predominantly biological processes underlying the natural de-astringency and “coagulation effect” in CPCNA. Nature Publishing Group 2017-03-17 /pmc/articles/PMC5356345/ /pubmed/28304376 http://dx.doi.org/10.1038/srep44671 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Wenxing
Xiong, Yalou
Xu, Liqing
Zhang, Qinglin
Luo, Zhengrong
An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon
title An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon
title_full An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon
title_fullStr An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon
title_full_unstemmed An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon
title_short An integrated analysis based on transcriptome and proteome reveals deastringency-related genes in CPCNA persimmon
title_sort integrated analysis based on transcriptome and proteome reveals deastringency-related genes in cpcna persimmon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356345/
https://www.ncbi.nlm.nih.gov/pubmed/28304376
http://dx.doi.org/10.1038/srep44671
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