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Histopathological changes in dental pulp of rats following radiotherapy

BACKGROUND: Radiotherapy is one of the oral and pharyngeal cancer treatment methods that can cause damage to the tissues in the radiation area; the purpose of this study is to evaluate the effects of radiotherapy on dental pulp tissue in rats. MATERIALS AND METHODS: In this interventional, experimen...

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Autores principales: Madani, Zahra Sadat, Azarakhsh, Soheil, Shakib, Pouyan Amini, Karimi, Masoomeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356384/
https://www.ncbi.nlm.nih.gov/pubmed/28348613
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author Madani, Zahra Sadat
Azarakhsh, Soheil
Shakib, Pouyan Amini
Karimi, Masoomeh
author_facet Madani, Zahra Sadat
Azarakhsh, Soheil
Shakib, Pouyan Amini
Karimi, Masoomeh
author_sort Madani, Zahra Sadat
collection PubMed
description BACKGROUND: Radiotherapy is one of the oral and pharyngeal cancer treatment methods that can cause damage to the tissues in the radiation area; the purpose of this study is to evaluate the effects of radiotherapy on dental pulp tissue in rats. MATERIALS AND METHODS: In this interventional, experimental double-blind study, 30 rats were studied in three groups (n = 10 each). The first group received 12 gray (Gy), the second group received 18 Gy in one session, and the third group was not exposed to radiation (control group). The 5 μm sections of mandibular molar tooth were prepared and stained with hematoxylin and eosin. Samples were studied under optical microscope to evaluate and score inflammation, necrosis, hyalinization, and vascular congestion. The data were coded and analyzed by statistical tests of χ(2) and Fisher's exact tests. The significant level of P = 0.05. RESULTS: In Group 1, necrosis in two cases, inflammation in one case, hyalinization in one case, and vascular congestion in four cases were observed. In Group 2, inflammation in four cases, hyalinization in two cases, and vascular congestion in five cases were observed. In Group 3, inflammation was observed only in one case. In comparison between the groups, no significant differences were observed in inflammation (P > 0.05), necrosis (P > 0.05), and hyalinization (P > 0.05). However, the difference was significant for vascular congestion (P < 0.05). CONCLUSION: Radiotherapy with doses of 12 and 18 Gy had no significant effect on inflammation, necrosis, and hyalinization in all groups; however, the difference was significant for vascular congestion.
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spelling pubmed-53563842017-03-27 Histopathological changes in dental pulp of rats following radiotherapy Madani, Zahra Sadat Azarakhsh, Soheil Shakib, Pouyan Amini Karimi, Masoomeh Dent Res J (Isfahan) Original Article BACKGROUND: Radiotherapy is one of the oral and pharyngeal cancer treatment methods that can cause damage to the tissues in the radiation area; the purpose of this study is to evaluate the effects of radiotherapy on dental pulp tissue in rats. MATERIALS AND METHODS: In this interventional, experimental double-blind study, 30 rats were studied in three groups (n = 10 each). The first group received 12 gray (Gy), the second group received 18 Gy in one session, and the third group was not exposed to radiation (control group). The 5 μm sections of mandibular molar tooth were prepared and stained with hematoxylin and eosin. Samples were studied under optical microscope to evaluate and score inflammation, necrosis, hyalinization, and vascular congestion. The data were coded and analyzed by statistical tests of χ(2) and Fisher's exact tests. The significant level of P = 0.05. RESULTS: In Group 1, necrosis in two cases, inflammation in one case, hyalinization in one case, and vascular congestion in four cases were observed. In Group 2, inflammation in four cases, hyalinization in two cases, and vascular congestion in five cases were observed. In Group 3, inflammation was observed only in one case. In comparison between the groups, no significant differences were observed in inflammation (P > 0.05), necrosis (P > 0.05), and hyalinization (P > 0.05). However, the difference was significant for vascular congestion (P < 0.05). CONCLUSION: Radiotherapy with doses of 12 and 18 Gy had no significant effect on inflammation, necrosis, and hyalinization in all groups; however, the difference was significant for vascular congestion. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5356384/ /pubmed/28348613 Text en Copyright: © 2017 Dental Research Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Madani, Zahra Sadat
Azarakhsh, Soheil
Shakib, Pouyan Amini
Karimi, Masoomeh
Histopathological changes in dental pulp of rats following radiotherapy
title Histopathological changes in dental pulp of rats following radiotherapy
title_full Histopathological changes in dental pulp of rats following radiotherapy
title_fullStr Histopathological changes in dental pulp of rats following radiotherapy
title_full_unstemmed Histopathological changes in dental pulp of rats following radiotherapy
title_short Histopathological changes in dental pulp of rats following radiotherapy
title_sort histopathological changes in dental pulp of rats following radiotherapy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356384/
https://www.ncbi.nlm.nih.gov/pubmed/28348613
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AT karimimasoomeh histopathologicalchangesindentalpulpofratsfollowingradiotherapy