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Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015

Multilocus variable-number tandem repeat analysis (MLVA) is a rapid and reproducible typing method that is an important tool for investigation, as well as detection, of national and multinational outbreaks of a range of food-borne pathogens. Salmonella enterica serovar Enteritidis is the most common...

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Autores principales: Peters, Tansy, Bertrand, Sophie, Björkman, Jonas T, Brandal, Lin T, Brown, Derek J, Erdõsi, Tímea, Heck, Max, Ibrahem, Salha, Johansson, Karin, Kornschober, Christian, Kotila, Saara M, Le Hello, Simon, Lienemann, Taru, Mattheus, Wesley, Nielsen, Eva Møller, Ragimbeau, Catherine, Rumore, Jillian, Sabol, Ashley, Torpdahl, Mia, Trees, Eija, Tuohy, Alma, de Pinna, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356433/
https://www.ncbi.nlm.nih.gov/pubmed/28277220
http://dx.doi.org/10.2807/1560-7917.ES.2017.22.9.30477
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author Peters, Tansy
Bertrand, Sophie
Björkman, Jonas T
Brandal, Lin T
Brown, Derek J
Erdõsi, Tímea
Heck, Max
Ibrahem, Salha
Johansson, Karin
Kornschober, Christian
Kotila, Saara M
Le Hello, Simon
Lienemann, Taru
Mattheus, Wesley
Nielsen, Eva Møller
Ragimbeau, Catherine
Rumore, Jillian
Sabol, Ashley
Torpdahl, Mia
Trees, Eija
Tuohy, Alma
de Pinna, Elizabeth
author_facet Peters, Tansy
Bertrand, Sophie
Björkman, Jonas T
Brandal, Lin T
Brown, Derek J
Erdõsi, Tímea
Heck, Max
Ibrahem, Salha
Johansson, Karin
Kornschober, Christian
Kotila, Saara M
Le Hello, Simon
Lienemann, Taru
Mattheus, Wesley
Nielsen, Eva Møller
Ragimbeau, Catherine
Rumore, Jillian
Sabol, Ashley
Torpdahl, Mia
Trees, Eija
Tuohy, Alma
de Pinna, Elizabeth
author_sort Peters, Tansy
collection PubMed
description Multilocus variable-number tandem repeat analysis (MLVA) is a rapid and reproducible typing method that is an important tool for investigation, as well as detection, of national and multinational outbreaks of a range of food-borne pathogens. Salmonella enterica serovar Enteritidis is the most common Salmonella serovar associated with human salmonellosis in the European Union/European Economic Area and North America. Fourteen laboratories from 13 countries in Europe and North America participated in a validation study for MLVA of S. Enteritidis targeting five loci. Following normalisation of fragment sizes using a set of reference strains, a blinded set of 24 strains with known allele sizes was analysed by each participant. The S. Enteritidis 5-loci MLVA protocol was shown to produce internationally comparable results as more than 90% of the participants reported less than 5% discrepant MLVA profiles. All 14 participating laboratories performed well, even those where experience with this typing method was limited. The raw fragment length data were consistent throughout, and the inter-laboratory validation helped to standardise the conversion of raw data to repeat numbers with at least two countries updating their internal procedures. However, differences in assigned MLVA profiles remain between well-established protocols and should be taken into account when exchanging data.
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spelling pubmed-53564332017-03-24 Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015 Peters, Tansy Bertrand, Sophie Björkman, Jonas T Brandal, Lin T Brown, Derek J Erdõsi, Tímea Heck, Max Ibrahem, Salha Johansson, Karin Kornschober, Christian Kotila, Saara M Le Hello, Simon Lienemann, Taru Mattheus, Wesley Nielsen, Eva Møller Ragimbeau, Catherine Rumore, Jillian Sabol, Ashley Torpdahl, Mia Trees, Eija Tuohy, Alma de Pinna, Elizabeth Euro Surveill Research Article Multilocus variable-number tandem repeat analysis (MLVA) is a rapid and reproducible typing method that is an important tool for investigation, as well as detection, of national and multinational outbreaks of a range of food-borne pathogens. Salmonella enterica serovar Enteritidis is the most common Salmonella serovar associated with human salmonellosis in the European Union/European Economic Area and North America. Fourteen laboratories from 13 countries in Europe and North America participated in a validation study for MLVA of S. Enteritidis targeting five loci. Following normalisation of fragment sizes using a set of reference strains, a blinded set of 24 strains with known allele sizes was analysed by each participant. The S. Enteritidis 5-loci MLVA protocol was shown to produce internationally comparable results as more than 90% of the participants reported less than 5% discrepant MLVA profiles. All 14 participating laboratories performed well, even those where experience with this typing method was limited. The raw fragment length data were consistent throughout, and the inter-laboratory validation helped to standardise the conversion of raw data to repeat numbers with at least two countries updating their internal procedures. However, differences in assigned MLVA profiles remain between well-established protocols and should be taken into account when exchanging data. European Centre for Disease Prevention and Control (ECDC) 2017-03-02 /pmc/articles/PMC5356433/ /pubmed/28277220 http://dx.doi.org/10.2807/1560-7917.ES.2017.22.9.30477 Text en This article is copyright of The Authors, 2017. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Research Article
Peters, Tansy
Bertrand, Sophie
Björkman, Jonas T
Brandal, Lin T
Brown, Derek J
Erdõsi, Tímea
Heck, Max
Ibrahem, Salha
Johansson, Karin
Kornschober, Christian
Kotila, Saara M
Le Hello, Simon
Lienemann, Taru
Mattheus, Wesley
Nielsen, Eva Møller
Ragimbeau, Catherine
Rumore, Jillian
Sabol, Ashley
Torpdahl, Mia
Trees, Eija
Tuohy, Alma
de Pinna, Elizabeth
Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
title Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
title_full Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
title_fullStr Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
title_full_unstemmed Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
title_short Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
title_sort multi-laboratory validation study of multilocus variable-number tandem repeat analysis (mlva) for salmonella enterica serovar enteritidis, 2015
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356433/
https://www.ncbi.nlm.nih.gov/pubmed/28277220
http://dx.doi.org/10.2807/1560-7917.ES.2017.22.9.30477
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