Cargando…
Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo
The expression patterns of the long non-coding RNA Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 (NNT-AS1) have not been investigated in the context of cancer. In this study, we aim to investigate the NNT-AS1 expression level in colorectal cancer (CRC) patients and its potential roles in t...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356894/ https://www.ncbi.nlm.nih.gov/pubmed/27966450 http://dx.doi.org/10.18632/oncotarget.13840 |
_version_ | 1782515941747195904 |
---|---|
author | Wang, Qian Yang, Lei Hu, Xin Jiang, Yuliang Hu, Yizhang Liu, Zhe Liu, Jian Wen, Tao Ma, Yingmin An, Guangyu Feng, Guosheng |
author_facet | Wang, Qian Yang, Lei Hu, Xin Jiang, Yuliang Hu, Yizhang Liu, Zhe Liu, Jian Wen, Tao Ma, Yingmin An, Guangyu Feng, Guosheng |
author_sort | Wang, Qian |
collection | PubMed |
description | The expression patterns of the long non-coding RNA Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 (NNT-AS1) have not been investigated in the context of cancer. In this study, we aim to investigate the NNT-AS1 expression level in colorectal cancer (CRC) patients and its potential roles in tumor biology. We measured the expression of NNT-AS1 in 70 paired tumor tissues and adjacent normal tissues. NNT-AS1 was expressed higher in tumor tissues than that in adjacent noncancer tissues, and higher expression of NNT-AS1 was significantly correlated with lymph node metastasis (Yes vs. No, P=0.004), TNM stage (I/II vs. III/IV, P=0.004), vessel invasion (Yes vs. No, P=0.002) and differentiation (well and moderate vs. poor, P=0.008). Multivariate analyses revealed that NNT-AS1 expression was an independent predictor of overall survival (P=0.0174) and progression free survival (P=0.0132) for CRC. Knockdown of NNT-AS1 using small interfering RNA (siRNA) significantly impaired CRC cell proliferation, migration and invasion in vitro and silencing NNT-AS1 also suppressed tumor growth and metastasis in nude mice. The western blot experiments revealed that silencing NNT-AS1 inhibited epithelial-mesenchymal transition (EMT) and inactivated MAPK/Erk signaling pathway in CRC cell lines. In conclusion, our studies implied that NNT-AS1 may involve in the development and progression of CRC via its regulation of cell proliferation, migration, and invasion by NNT-AS1-mediated activating of MAPK/Erk signaling pathway and EMT. NNT-AS1 may be a useful diagnostic and prognostic biomarker and a potential therapeutic target in CRC patients. |
format | Online Article Text |
id | pubmed-5356894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53568942017-04-20 Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo Wang, Qian Yang, Lei Hu, Xin Jiang, Yuliang Hu, Yizhang Liu, Zhe Liu, Jian Wen, Tao Ma, Yingmin An, Guangyu Feng, Guosheng Oncotarget Research Paper The expression patterns of the long non-coding RNA Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 (NNT-AS1) have not been investigated in the context of cancer. In this study, we aim to investigate the NNT-AS1 expression level in colorectal cancer (CRC) patients and its potential roles in tumor biology. We measured the expression of NNT-AS1 in 70 paired tumor tissues and adjacent normal tissues. NNT-AS1 was expressed higher in tumor tissues than that in adjacent noncancer tissues, and higher expression of NNT-AS1 was significantly correlated with lymph node metastasis (Yes vs. No, P=0.004), TNM stage (I/II vs. III/IV, P=0.004), vessel invasion (Yes vs. No, P=0.002) and differentiation (well and moderate vs. poor, P=0.008). Multivariate analyses revealed that NNT-AS1 expression was an independent predictor of overall survival (P=0.0174) and progression free survival (P=0.0132) for CRC. Knockdown of NNT-AS1 using small interfering RNA (siRNA) significantly impaired CRC cell proliferation, migration and invasion in vitro and silencing NNT-AS1 also suppressed tumor growth and metastasis in nude mice. The western blot experiments revealed that silencing NNT-AS1 inhibited epithelial-mesenchymal transition (EMT) and inactivated MAPK/Erk signaling pathway in CRC cell lines. In conclusion, our studies implied that NNT-AS1 may involve in the development and progression of CRC via its regulation of cell proliferation, migration, and invasion by NNT-AS1-mediated activating of MAPK/Erk signaling pathway and EMT. NNT-AS1 may be a useful diagnostic and prognostic biomarker and a potential therapeutic target in CRC patients. Impact Journals LLC 2016-12-09 /pmc/articles/PMC5356894/ /pubmed/27966450 http://dx.doi.org/10.18632/oncotarget.13840 Text en Copyright: © 2017 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Qian Yang, Lei Hu, Xin Jiang, Yuliang Hu, Yizhang Liu, Zhe Liu, Jian Wen, Tao Ma, Yingmin An, Guangyu Feng, Guosheng Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
title | Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
title_full | Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
title_fullStr | Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
title_full_unstemmed | Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
title_short | Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
title_sort | upregulated nnt-as1, a long noncoding rna, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356894/ https://www.ncbi.nlm.nih.gov/pubmed/27966450 http://dx.doi.org/10.18632/oncotarget.13840 |
work_keys_str_mv | AT wangqian upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT yanglei upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT huxin upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT jiangyuliang upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT huyizhang upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT liuzhe upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT liujian upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT wentao upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT mayingmin upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT anguangyu upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo AT fengguosheng upregulatednntas1alongnoncodingrnacontributestoproliferationandmigrationofcolorectalcancercellsinvitroandinvivo |