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Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts
Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous e...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357111/ https://www.ncbi.nlm.nih.gov/pubmed/28344485 http://dx.doi.org/10.1016/j.jsps.2016.06.008 |
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author | Coballase-Urrutia, Elvia Cárdenas-Rodríguez, Noemí González-García, María Carolina Núñez-Ramírez, Eithan Floriano-Sánchez, Esaú González-Trujano, María Eva Fernández-Rojas, Berenice Pedraza-Chaverrí, José Montesinos-Correa, Hortencia Rivera-Espinosa, Liliana Sampieri, Aristides III Carmona-Aparicio, Liliana |
author_facet | Coballase-Urrutia, Elvia Cárdenas-Rodríguez, Noemí González-García, María Carolina Núñez-Ramírez, Eithan Floriano-Sánchez, Esaú González-Trujano, María Eva Fernández-Rojas, Berenice Pedraza-Chaverrí, José Montesinos-Correa, Hortencia Rivera-Espinosa, Liliana Sampieri, Aristides III Carmona-Aparicio, Liliana |
author_sort | Coballase-Urrutia, Elvia |
collection | PubMed |
description | Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p.) obtained from the leaves of Tilia americana var. mexicana on CCl(4)-induced liver and brain damage in the rat. Protection was observed in the liver and brain (cerebellum, cortex and cerebral hemispheres) by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA) using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl(4)-treated rats. The T. americana var. mexicana leaf extracts provided significant protection against CCl(4)-induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-globulin (γ-GLOB), serum albumin (ALB), total bilirubin (BB), creatinine (CREA) and creatine kinase (CK), relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous Tilia extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of T. americana var. mexicana that confer its neuro- and hepatoprotective effects. |
format | Online Article Text |
id | pubmed-5357111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53571112017-03-24 Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts Coballase-Urrutia, Elvia Cárdenas-Rodríguez, Noemí González-García, María Carolina Núñez-Ramírez, Eithan Floriano-Sánchez, Esaú González-Trujano, María Eva Fernández-Rojas, Berenice Pedraza-Chaverrí, José Montesinos-Correa, Hortencia Rivera-Espinosa, Liliana Sampieri, Aristides III Carmona-Aparicio, Liliana Saudi Pharm J Original Article Around the world, species from the genus Tilia are commonly used because of their peripheral and central medicinal effects; they are prepared as teas and used as tranquilizing, anticonvulsant, and analgesic agents. In this study, we provide evidence of the protective effects of organic and aqueous extracts (100 mg/kg, i.p.) obtained from the leaves of Tilia americana var. mexicana on CCl(4)-induced liver and brain damage in the rat. Protection was observed in the liver and brain (cerebellum, cortex and cerebral hemispheres) by measuring the activity of antioxidant enzymes and levels of malondialdehyde (MDA) using spectrophotometric methods. Biochemical parameters were also assessed in serum samples from the CCl(4)-treated rats. The T. americana var. mexicana leaf extracts provided significant protection against CCl(4)-induced peripheral and central damage by increasing the activity of antioxidant enzymes, diminishing lipid peroxidation, and preventing alterations in biochemical serum parameters, such as the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-globulin (γ-GLOB), serum albumin (ALB), total bilirubin (BB), creatinine (CREA) and creatine kinase (CK), relative to the control group. Additionally, we correlated gene expression with antioxidant activity in the experimental groups treated with the organic and aqueous Tilia extracts and observed a non-statistically significant positive correlation. Our results provide evidence of the underlying biomedical properties of T. americana var. mexicana that confer its neuro- and hepatoprotective effects. Elsevier 2017-03 2016-07-12 /pmc/articles/PMC5357111/ /pubmed/28344485 http://dx.doi.org/10.1016/j.jsps.2016.06.008 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Coballase-Urrutia, Elvia Cárdenas-Rodríguez, Noemí González-García, María Carolina Núñez-Ramírez, Eithan Floriano-Sánchez, Esaú González-Trujano, María Eva Fernández-Rojas, Berenice Pedraza-Chaverrí, José Montesinos-Correa, Hortencia Rivera-Espinosa, Liliana Sampieri, Aristides III Carmona-Aparicio, Liliana Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts |
title | Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts |
title_full | Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts |
title_fullStr | Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts |
title_full_unstemmed | Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts |
title_short | Biochemical and molecular modulation of CCl(4)-induced peripheral and central damage by Tilia americana var. mexicanaextracts |
title_sort | biochemical and molecular modulation of ccl(4)-induced peripheral and central damage by tilia americana var. mexicanaextracts |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357111/ https://www.ncbi.nlm.nih.gov/pubmed/28344485 http://dx.doi.org/10.1016/j.jsps.2016.06.008 |
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