Perfused Gills Reveal Fundamental Principles of pH Regulation and Ammonia Homeostasis in the Cephalopod Octopus vulgaris

In contrast to terrestrial animals most aquatic species can be characterized by relatively higher blood [Formula: see text] concentrations despite its potential toxicity to the central nervous system. Although many aquatic species excrete [Formula: see text] via specialized epithelia little information is available regarding the mechanistic basis for NH(3)/ [Formula: see text] homeostasis in molluscs. Using perfused gills of Octopus vulgaris we studied acid-base regulation and ammonia excretion pathways in this cephalopod species. The octopus gill is capable of regulating ammonia (NH(3)/ [Formula: see text]) homeostasis by the accumulation of ammonia at low blood levels (<260 μM) and secretion at blood ammonia concentrations exceeding in vivo levels of 300 μM. [Formula: see text] transport is sensitive to the adenylyl cyclase inhibitor KH7 indicating that this process is mediated through cAMP-dependent pathways. The perfused octopus gill has substantial pH regulatory abilities during an acidosis, accompanied by an increased secretion of [Formula: see text]. Immunohistochemical and qPCR analyses revealed tissue specific expression and localization of Na(+)/K(+)-ATPase, V-type H(+)-ATPase, Na(+)/H(+)-exchanger 3, and Rhesus protein in the gill. Using the octopus gill as a molluscan model, our results highlight the coupling of acid-base regulation and nitrogen excretion, which may represent a conserved pH regulatory mechanism across many marine taxa.