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The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features

BACKGROUND: Early studies established that certain lipids were lower in acute myeloid leukemia (AML) cells than normal leukocytes. Because lipids are now known to play an important role in cell signaling and regulation of homeostasis, and are often perturbed in malignancies, we undertook a comprehen...

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Autores principales: Pabst, Thomas, Kortz, Linda, Fiedler, Georg M., Ceglarek, Uta, Idle, Jeffrey R., Beyoğlu, Diren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357680/
https://www.ncbi.nlm.nih.gov/pubmed/28331812
http://dx.doi.org/10.1016/j.bbacli.2017.03.002
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author Pabst, Thomas
Kortz, Linda
Fiedler, Georg M.
Ceglarek, Uta
Idle, Jeffrey R.
Beyoğlu, Diren
author_facet Pabst, Thomas
Kortz, Linda
Fiedler, Georg M.
Ceglarek, Uta
Idle, Jeffrey R.
Beyoğlu, Diren
author_sort Pabst, Thomas
collection PubMed
description BACKGROUND: Early studies established that certain lipids were lower in acute myeloid leukemia (AML) cells than normal leukocytes. Because lipids are now known to play an important role in cell signaling and regulation of homeostasis, and are often perturbed in malignancies, we undertook a comprehensive lipidomic survey of plasma from AML patients at time of diagnosis and also healthy blood donors. METHODS: Plasma lipid profiles were measured using three mass spectrometry platforms in 20 AML patients and 20 healthy blood donors. Data were collected on total cholesterol and fatty acids, fatty acid amides, glycerolipids, phospholipids, sphingolipids, cholesterol esters, coenzyme Q10 and eicosanoids. RESULTS: We observed a depletion of plasma total fatty acids and cholesterol, but an increase in certain free fatty acids with the observed decline in sphingolipids, phosphocholines, triglycerides and cholesterol esters probably driven by enhanced fatty acid oxidation in AML cells. Arachidonic acid and precursors were elevated in AML, particularly in patients with high bone marrow (BM) or peripheral blasts and unfavorable prognostic risk. PGF2α was also elevated, in patients with low BM or peripheral blasts and with a favorable prognostic risk. A broad panoply of lipid classes is altered in AML plasma, pointing to disturbances of several lipid metabolic interconversions, in particular in relation to blast cell counts and prognostic risk. CONCLUSIONS: These data indicate potential roles played by lipids in AML heterogeneity and disease outcome. GENERAL SIGNIFICANCE: Enhanced catabolism of several lipid classes increases prognostic risk while plasma PGF2α may be a marker for reduced prognostic risk in AML.
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spelling pubmed-53576802017-03-22 The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features Pabst, Thomas Kortz, Linda Fiedler, Georg M. Ceglarek, Uta Idle, Jeffrey R. Beyoğlu, Diren BBA Clin Regular Article BACKGROUND: Early studies established that certain lipids were lower in acute myeloid leukemia (AML) cells than normal leukocytes. Because lipids are now known to play an important role in cell signaling and regulation of homeostasis, and are often perturbed in malignancies, we undertook a comprehensive lipidomic survey of plasma from AML patients at time of diagnosis and also healthy blood donors. METHODS: Plasma lipid profiles were measured using three mass spectrometry platforms in 20 AML patients and 20 healthy blood donors. Data were collected on total cholesterol and fatty acids, fatty acid amides, glycerolipids, phospholipids, sphingolipids, cholesterol esters, coenzyme Q10 and eicosanoids. RESULTS: We observed a depletion of plasma total fatty acids and cholesterol, but an increase in certain free fatty acids with the observed decline in sphingolipids, phosphocholines, triglycerides and cholesterol esters probably driven by enhanced fatty acid oxidation in AML cells. Arachidonic acid and precursors were elevated in AML, particularly in patients with high bone marrow (BM) or peripheral blasts and unfavorable prognostic risk. PGF2α was also elevated, in patients with low BM or peripheral blasts and with a favorable prognostic risk. A broad panoply of lipid classes is altered in AML plasma, pointing to disturbances of several lipid metabolic interconversions, in particular in relation to blast cell counts and prognostic risk. CONCLUSIONS: These data indicate potential roles played by lipids in AML heterogeneity and disease outcome. GENERAL SIGNIFICANCE: Enhanced catabolism of several lipid classes increases prognostic risk while plasma PGF2α may be a marker for reduced prognostic risk in AML. Elsevier 2017-03-08 /pmc/articles/PMC5357680/ /pubmed/28331812 http://dx.doi.org/10.1016/j.bbacli.2017.03.002 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Pabst, Thomas
Kortz, Linda
Fiedler, Georg M.
Ceglarek, Uta
Idle, Jeffrey R.
Beyoğlu, Diren
The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
title The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
title_full The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
title_fullStr The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
title_full_unstemmed The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
title_short The plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
title_sort plasma lipidome in acute myeloid leukemia at diagnosis in relation to clinical disease features
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357680/
https://www.ncbi.nlm.nih.gov/pubmed/28331812
http://dx.doi.org/10.1016/j.bbacli.2017.03.002
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