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EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI

BACKGROUND: Escherichia coli is the agent responsible for a range of clinical diseases. With emerging antimicrobial resistance, other treatment options including solar/photo-therapy are becoming increasingly common. Visible Range Radiation Therapy/Colour Therapy is an emerging technique in the field...

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Autores principales: Azeemi, Samina T. Yousuf, Shaukat, Saleem Farooq, Azeemi, Khawaja Shamsuddin, Khan, Idrees, Mahmood, Khalid, Naz, Farah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357883/
https://www.ncbi.nlm.nih.gov/pubmed/28331912
http://dx.doi.org/10.21010/ajtcam.v14i1.4
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author Azeemi, Samina T. Yousuf
Shaukat, Saleem Farooq
Azeemi, Khawaja Shamsuddin
Khan, Idrees
Mahmood, Khalid
Naz, Farah
author_facet Azeemi, Samina T. Yousuf
Shaukat, Saleem Farooq
Azeemi, Khawaja Shamsuddin
Khan, Idrees
Mahmood, Khalid
Naz, Farah
author_sort Azeemi, Samina T. Yousuf
collection PubMed
description BACKGROUND: Escherichia coli is the agent responsible for a range of clinical diseases. With emerging antimicrobial resistance, other treatment options including solar/photo-therapy are becoming increasingly common. Visible Range Radiation Therapy/Colour Therapy is an emerging technique in the field of energy/vibrational medicine that uses visible spectrum of Electromagnetic Radiations to cure different diseases. In this study, our goal was to understand the effect of Visible Range Electromagnetic Radiations on E. coli (in vitro) and therefore find out the most appropriate visible range radiation for the treatment of diseases caused by E. coli. MATERIALS AND METHODS: A total of 6 non-repetitive E. coli isolates were obtained from urine samples obtained from hospitalized patients with UTI. Single colony of E. coli was inoculated in 3 ml of Lysogeny Broth (LB) and 40 μl of this E. coli suspension was poured into each of the plastic tubes which were then irradiated with six different wavelengths in the visible region (Table. 1) after 18 hours with one acting as a control. The Optical Densities of these irradiated samples were then measured. Furthermore, scanning electron microscopy (TEFCAN ZEGA3) was carried out. RESULTS: The analysis of the microscopic and SEM images of irradiated E. coli samples with six different visible range radiations is representative of The fact that E. coli responded differently to every applied radiation in the visible region and the most profound inhibitory effects were that of 538nm Visible Range Radiation (Green) which proved to be bactericidal and 590nm Visible Range Radiation (yellow) which was bacteriostatic. The enhanced growth of E. coli with varying degrees was clearly observed in 610nm (orange), 644nm (red), 464nm (Purple) and 453nm (blue). CONCLUSION: It can be concluded that 538nm (Green) and 590nm (Yellow) can effectively be used for treating E. coli borne diseases.
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spelling pubmed-53578832017-06-05 EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI Azeemi, Samina T. Yousuf Shaukat, Saleem Farooq Azeemi, Khawaja Shamsuddin Khan, Idrees Mahmood, Khalid Naz, Farah Afr J Tradit Complement Altern Med Article BACKGROUND: Escherichia coli is the agent responsible for a range of clinical diseases. With emerging antimicrobial resistance, other treatment options including solar/photo-therapy are becoming increasingly common. Visible Range Radiation Therapy/Colour Therapy is an emerging technique in the field of energy/vibrational medicine that uses visible spectrum of Electromagnetic Radiations to cure different diseases. In this study, our goal was to understand the effect of Visible Range Electromagnetic Radiations on E. coli (in vitro) and therefore find out the most appropriate visible range radiation for the treatment of diseases caused by E. coli. MATERIALS AND METHODS: A total of 6 non-repetitive E. coli isolates were obtained from urine samples obtained from hospitalized patients with UTI. Single colony of E. coli was inoculated in 3 ml of Lysogeny Broth (LB) and 40 μl of this E. coli suspension was poured into each of the plastic tubes which were then irradiated with six different wavelengths in the visible region (Table. 1) after 18 hours with one acting as a control. The Optical Densities of these irradiated samples were then measured. Furthermore, scanning electron microscopy (TEFCAN ZEGA3) was carried out. RESULTS: The analysis of the microscopic and SEM images of irradiated E. coli samples with six different visible range radiations is representative of The fact that E. coli responded differently to every applied radiation in the visible region and the most profound inhibitory effects were that of 538nm Visible Range Radiation (Green) which proved to be bactericidal and 590nm Visible Range Radiation (yellow) which was bacteriostatic. The enhanced growth of E. coli with varying degrees was clearly observed in 610nm (orange), 644nm (red), 464nm (Purple) and 453nm (blue). CONCLUSION: It can be concluded that 538nm (Green) and 590nm (Yellow) can effectively be used for treating E. coli borne diseases. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2016-11-23 /pmc/articles/PMC5357883/ /pubmed/28331912 http://dx.doi.org/10.21010/ajtcam.v14i1.4 Text en Copyright: © 2017 Afr. J. Traditional Complementary and Alternative Medicines http://creativecommons.org/licenses/CC-BY/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Article
Azeemi, Samina T. Yousuf
Shaukat, Saleem Farooq
Azeemi, Khawaja Shamsuddin
Khan, Idrees
Mahmood, Khalid
Naz, Farah
EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
title EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
title_full EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
title_fullStr EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
title_full_unstemmed EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
title_short EFFECT OF VISIBLE RANGE ELECTROMAGNETIC RADIATIONS ON ESCHERICHIA COLI
title_sort effect of visible range electromagnetic radiations on escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357883/
https://www.ncbi.nlm.nih.gov/pubmed/28331912
http://dx.doi.org/10.21010/ajtcam.v14i1.4
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