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Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103

More and more new natural products have been found in Streptomyces species, which become the significant resource for antibiotics production. Among them, Streptomyces lydicus has been known as its ability of streptolydigin biosynthesis. Herein, we present the genome analysis of S. lydicus based on t...

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Autores principales: Jia, Nan, Ding, Ming-Zhu, Luo, Hao, Gao, Feng, Yuan, Ying-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357945/
https://www.ncbi.nlm.nih.gov/pubmed/28317865
http://dx.doi.org/10.1038/srep44786
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author Jia, Nan
Ding, Ming-Zhu
Luo, Hao
Gao, Feng
Yuan, Ying-Jin
author_facet Jia, Nan
Ding, Ming-Zhu
Luo, Hao
Gao, Feng
Yuan, Ying-Jin
author_sort Jia, Nan
collection PubMed
description More and more new natural products have been found in Streptomyces species, which become the significant resource for antibiotics production. Among them, Streptomyces lydicus has been known as its ability of streptolydigin biosynthesis. Herein, we present the genome analysis of S. lydicus based on the complete genome sequencing. The circular chromosome of S. lydicus 103 comprises 8,201,357 base pairs with average GC content 72.22%. With the aid of KEGG analysis, we found that S. lydicus 103 can transfer propanoate to succinate, glutamine or glutamate to 2-oxoglutarate, CO(2) and L-glutamate to ammonia, which are conducive to the the supply of amino acids. S. lydicus 103 encodes acyl-CoA thioesterase II that takes part in biosynthesis of unsaturated fatty acids, and harbors the complete biosynthesis pathways of lysine, valine, leucine, phenylalanine, tyrosine and isoleucine. Furthermore, a total of 27 putative gene clusters have been predicted to be involved in secondary metabolism, including biosynthesis of streptolydigin, erythromycin, mannopeptimycin, ectoine and desferrioxamine B. Comparative genome analysis of S. lydicus 103 will help us deeply understand its metabolic pathways, which is essential for enhancing the antibiotic production through metabolic engineering.
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spelling pubmed-53579452017-03-22 Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103 Jia, Nan Ding, Ming-Zhu Luo, Hao Gao, Feng Yuan, Ying-Jin Sci Rep Article More and more new natural products have been found in Streptomyces species, which become the significant resource for antibiotics production. Among them, Streptomyces lydicus has been known as its ability of streptolydigin biosynthesis. Herein, we present the genome analysis of S. lydicus based on the complete genome sequencing. The circular chromosome of S. lydicus 103 comprises 8,201,357 base pairs with average GC content 72.22%. With the aid of KEGG analysis, we found that S. lydicus 103 can transfer propanoate to succinate, glutamine or glutamate to 2-oxoglutarate, CO(2) and L-glutamate to ammonia, which are conducive to the the supply of amino acids. S. lydicus 103 encodes acyl-CoA thioesterase II that takes part in biosynthesis of unsaturated fatty acids, and harbors the complete biosynthesis pathways of lysine, valine, leucine, phenylalanine, tyrosine and isoleucine. Furthermore, a total of 27 putative gene clusters have been predicted to be involved in secondary metabolism, including biosynthesis of streptolydigin, erythromycin, mannopeptimycin, ectoine and desferrioxamine B. Comparative genome analysis of S. lydicus 103 will help us deeply understand its metabolic pathways, which is essential for enhancing the antibiotic production through metabolic engineering. Nature Publishing Group 2017-03-20 /pmc/articles/PMC5357945/ /pubmed/28317865 http://dx.doi.org/10.1038/srep44786 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jia, Nan
Ding, Ming-Zhu
Luo, Hao
Gao, Feng
Yuan, Ying-Jin
Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
title Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
title_full Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
title_fullStr Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
title_full_unstemmed Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
title_short Complete genome sequencing and antibiotics biosynthesis pathways analysis of Streptomyces lydicus 103
title_sort complete genome sequencing and antibiotics biosynthesis pathways analysis of streptomyces lydicus 103
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357945/
https://www.ncbi.nlm.nih.gov/pubmed/28317865
http://dx.doi.org/10.1038/srep44786
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