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Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions

Enterohemorrhagic Escherichia coli (EHEC) are major food-borne pathogens whose survival and virulence in the human digestive tract remain unclear owing to paucity of relevant models. EHEC interact with the follicle-associated epithelium of Peyer’s patches of the distal ileum and translocate across t...

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Autores principales: Cordonnier, Charlotte, Etienne-Mesmin, Lucie, Thévenot, Jonathan, Rougeron, Amandine, Rénier, Sandra, Chassaing, Benoit, Darfeuille-Michaud, Arlette, Barnich, Nicolas, Blanquet-Diot, Stéphanie, Livrelli, Valérie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357955/
https://www.ncbi.nlm.nih.gov/pubmed/28317910
http://dx.doi.org/10.1038/srep44655
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author Cordonnier, Charlotte
Etienne-Mesmin, Lucie
Thévenot, Jonathan
Rougeron, Amandine
Rénier, Sandra
Chassaing, Benoit
Darfeuille-Michaud, Arlette
Barnich, Nicolas
Blanquet-Diot, Stéphanie
Livrelli, Valérie
author_facet Cordonnier, Charlotte
Etienne-Mesmin, Lucie
Thévenot, Jonathan
Rougeron, Amandine
Rénier, Sandra
Chassaing, Benoit
Darfeuille-Michaud, Arlette
Barnich, Nicolas
Blanquet-Diot, Stéphanie
Livrelli, Valérie
author_sort Cordonnier, Charlotte
collection PubMed
description Enterohemorrhagic Escherichia coli (EHEC) are major food-borne pathogens whose survival and virulence in the human digestive tract remain unclear owing to paucity of relevant models. EHEC interact with the follicle-associated epithelium of Peyer’s patches of the distal ileum and translocate across the intestinal epithelium via M-cells, but the underlying molecular mechanisms are still unknown. Here, we investigated the involvement of Long polar fimbriae (Lpf) in EHEC pathogenesis. Of the 236 strains tested, a significant association was observed between the presence of lpf operons and pathogenicity. In sophisticated in vitro models of the human gastro-intestinal tract, lpf expression was induced during transit through the simulated stomach and small intestine, but not in the colonic compartment. To investigate the involvement of Lpf in EHEC pathogenesis, lpf isogenic mutants and their relative trans-complemented strains were generated. Translocation across M-cells, interactions with murine ileal biopsies containing Peyer’s patches and the number of hemorrhagic lesions were significantly reduced with the lpf mutants compared to the wild-type strain. Complementation of lpf mutants fully restored the wild-type phenotypes. Our results indicate that (i) EHEC might colonize the terminal ileum at the early stages of infection, (ii) Lpf are an important player in the interactions with Peyer’s patches and M-cells, and could contribute to intestinal colonization.
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spelling pubmed-53579552017-03-22 Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions Cordonnier, Charlotte Etienne-Mesmin, Lucie Thévenot, Jonathan Rougeron, Amandine Rénier, Sandra Chassaing, Benoit Darfeuille-Michaud, Arlette Barnich, Nicolas Blanquet-Diot, Stéphanie Livrelli, Valérie Sci Rep Article Enterohemorrhagic Escherichia coli (EHEC) are major food-borne pathogens whose survival and virulence in the human digestive tract remain unclear owing to paucity of relevant models. EHEC interact with the follicle-associated epithelium of Peyer’s patches of the distal ileum and translocate across the intestinal epithelium via M-cells, but the underlying molecular mechanisms are still unknown. Here, we investigated the involvement of Long polar fimbriae (Lpf) in EHEC pathogenesis. Of the 236 strains tested, a significant association was observed between the presence of lpf operons and pathogenicity. In sophisticated in vitro models of the human gastro-intestinal tract, lpf expression was induced during transit through the simulated stomach and small intestine, but not in the colonic compartment. To investigate the involvement of Lpf in EHEC pathogenesis, lpf isogenic mutants and their relative trans-complemented strains were generated. Translocation across M-cells, interactions with murine ileal biopsies containing Peyer’s patches and the number of hemorrhagic lesions were significantly reduced with the lpf mutants compared to the wild-type strain. Complementation of lpf mutants fully restored the wild-type phenotypes. Our results indicate that (i) EHEC might colonize the terminal ileum at the early stages of infection, (ii) Lpf are an important player in the interactions with Peyer’s patches and M-cells, and could contribute to intestinal colonization. Nature Publishing Group 2017-03-20 /pmc/articles/PMC5357955/ /pubmed/28317910 http://dx.doi.org/10.1038/srep44655 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Cordonnier, Charlotte
Etienne-Mesmin, Lucie
Thévenot, Jonathan
Rougeron, Amandine
Rénier, Sandra
Chassaing, Benoit
Darfeuille-Michaud, Arlette
Barnich, Nicolas
Blanquet-Diot, Stéphanie
Livrelli, Valérie
Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions
title Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions
title_full Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions
title_fullStr Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions
title_full_unstemmed Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions
title_short Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer’s patches interactions
title_sort enterohemorrhagic escherichia coli pathogenesis: role of long polar fimbriae in peyer’s patches interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357955/
https://www.ncbi.nlm.nih.gov/pubmed/28317910
http://dx.doi.org/10.1038/srep44655
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