Reversible DNA micro-patterning using the fluorous effect

We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency...

Descripción completa

Detalles Bibliográficos
Autores principales: Flynn, Gabriella E., Withers, Jamie M., Macias, Gerard, Sperling, Justin R., Henry, Sarah L., Cooper, Jonathan M., Burley, Glenn A., Clark, Alasdair W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358500/
https://www.ncbi.nlm.nih.gov/pubmed/28243661
http://dx.doi.org/10.1039/c7cc00288b
_version_ 1782516239917121536
author Flynn, Gabriella E.
Withers, Jamie M.
Macias, Gerard
Sperling, Justin R.
Henry, Sarah L.
Cooper, Jonathan M.
Burley, Glenn A.
Clark, Alasdair W.
author_facet Flynn, Gabriella E.
Withers, Jamie M.
Macias, Gerard
Sperling, Justin R.
Henry, Sarah L.
Cooper, Jonathan M.
Burley, Glenn A.
Clark, Alasdair W.
author_sort Flynn, Gabriella E.
collection PubMed
description We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.
format Online
Article
Text
id pubmed-5358500
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-53585002017-05-15 Reversible DNA micro-patterning using the fluorous effect Flynn, Gabriella E. Withers, Jamie M. Macias, Gerard Sperling, Justin R. Henry, Sarah L. Cooper, Jonathan M. Burley, Glenn A. Clark, Alasdair W. Chem Commun (Camb) Chemistry We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences. Royal Society of Chemistry 2017-03-14 2017-02-23 /pmc/articles/PMC5358500/ /pubmed/28243661 http://dx.doi.org/10.1039/c7cc00288b Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Flynn, Gabriella E.
Withers, Jamie M.
Macias, Gerard
Sperling, Justin R.
Henry, Sarah L.
Cooper, Jonathan M.
Burley, Glenn A.
Clark, Alasdair W.
Reversible DNA micro-patterning using the fluorous effect
title Reversible DNA micro-patterning using the fluorous effect
title_full Reversible DNA micro-patterning using the fluorous effect
title_fullStr Reversible DNA micro-patterning using the fluorous effect
title_full_unstemmed Reversible DNA micro-patterning using the fluorous effect
title_short Reversible DNA micro-patterning using the fluorous effect
title_sort reversible dna micro-patterning using the fluorous effect
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358500/
https://www.ncbi.nlm.nih.gov/pubmed/28243661
http://dx.doi.org/10.1039/c7cc00288b
work_keys_str_mv AT flynngabriellae reversiblednamicropatterningusingthefluorouseffect
AT withersjamiem reversiblednamicropatterningusingthefluorouseffect
AT maciasgerard reversiblednamicropatterningusingthefluorouseffect
AT sperlingjustinr reversiblednamicropatterningusingthefluorouseffect
AT henrysarahl reversiblednamicropatterningusingthefluorouseffect
AT cooperjonathanm reversiblednamicropatterningusingthefluorouseffect
AT burleyglenna reversiblednamicropatterningusingthefluorouseffect
AT clarkalasdairw reversiblednamicropatterningusingthefluorouseffect

Ejemplares similares