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Affinity purification of human m-calpain through an intrinsically disordered inhibitor, calpastatin
Calpains are calcium-activated proteases that have biomedical and biotechnological potential. Their activity is tightly regulated by their endogenous inhibitor, calpastatin that binds to the enzyme only in the presence of calcium. Conventional approaches to purify calpain comprise multiple chromatog...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358782/ https://www.ncbi.nlm.nih.gov/pubmed/28319173 http://dx.doi.org/10.1371/journal.pone.0174125 |