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Transcriptional oscillation of canonical clock genes in mouse peripheral tissues
BACKGROUND: The circadian rhythm of about 24 hours is a fundamental physiological function observed in almost all organisms from prokaryotes to humans. Identification of clock genes has allowed us to study the molecular bases for circadian behaviors and temporal physiological processes such as hormo...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2004
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC535906/ https://www.ncbi.nlm.nih.gov/pubmed/15473909 http://dx.doi.org/10.1186/1471-2199-5-18 |
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author | Yamamoto, Takuro Nakahata, Yasukazu Soma, Haruhiko Akashi, Makoto Mamine, Takayoshi Takumi, Toru |
author_facet | Yamamoto, Takuro Nakahata, Yasukazu Soma, Haruhiko Akashi, Makoto Mamine, Takayoshi Takumi, Toru |
author_sort | Yamamoto, Takuro |
collection | PubMed |
description | BACKGROUND: The circadian rhythm of about 24 hours is a fundamental physiological function observed in almost all organisms from prokaryotes to humans. Identification of clock genes has allowed us to study the molecular bases for circadian behaviors and temporal physiological processes such as hormonal secretion, and has prompted the idea that molecular clocks reside not only in a central pacemaker, the suprachiasmatic nuclei (SCN) of hypothalamus in mammals, but also in peripheral tissues, even in immortalized cells. Furthermore, previous molecular dissection revealed that the mechanism of circadian oscillation at a molecular level is based on transcriptional regulation of clock and clock-controlled genes. RESULTS: We systematically analyzed the mRNA expression of clock and clock-controlled genes in mouse peripheral tissues. Eight genes (mBmal1, mNpas2, mRev-erbα, mDbp, mRev-erbβ, mPer3, mPer1 and mPer2; given in the temporal order of the rhythm peak) showed robust circadian expressions of mRNAs in all tissues except testis, suggesting that these genes are core molecules of the molecular biological clock. The bioinformatics analysis revealed that these genes have one or a combination of 3 transcriptional elements (RORE, DBPE, and E-box), which are conserved among human, mouse, and rat genome sequences, and indicated that these 3 elements may be responsible for the biological timing of expression of canonical clock genes. CONCLUSIONS: The observation of oscillatory profiles of canonical clock genes is not only useful for physiological and pathological examination of the circadian clock in various organs but also important for systematic understanding of transcriptional regulation on a genome-wide basis. Our finding of the oscillatory expression of canonical clock genes with a temporal order provides us an interesting hypothesis, that cyclic timing of all clock and clock-controlled genes may be dependent on several transcriptional elements including 3 known elements, E-box, RORE, and DBPE. |
format | Text |
id | pubmed-535906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-5359062004-12-17 Transcriptional oscillation of canonical clock genes in mouse peripheral tissues Yamamoto, Takuro Nakahata, Yasukazu Soma, Haruhiko Akashi, Makoto Mamine, Takayoshi Takumi, Toru BMC Mol Biol Research Article BACKGROUND: The circadian rhythm of about 24 hours is a fundamental physiological function observed in almost all organisms from prokaryotes to humans. Identification of clock genes has allowed us to study the molecular bases for circadian behaviors and temporal physiological processes such as hormonal secretion, and has prompted the idea that molecular clocks reside not only in a central pacemaker, the suprachiasmatic nuclei (SCN) of hypothalamus in mammals, but also in peripheral tissues, even in immortalized cells. Furthermore, previous molecular dissection revealed that the mechanism of circadian oscillation at a molecular level is based on transcriptional regulation of clock and clock-controlled genes. RESULTS: We systematically analyzed the mRNA expression of clock and clock-controlled genes in mouse peripheral tissues. Eight genes (mBmal1, mNpas2, mRev-erbα, mDbp, mRev-erbβ, mPer3, mPer1 and mPer2; given in the temporal order of the rhythm peak) showed robust circadian expressions of mRNAs in all tissues except testis, suggesting that these genes are core molecules of the molecular biological clock. The bioinformatics analysis revealed that these genes have one or a combination of 3 transcriptional elements (RORE, DBPE, and E-box), which are conserved among human, mouse, and rat genome sequences, and indicated that these 3 elements may be responsible for the biological timing of expression of canonical clock genes. CONCLUSIONS: The observation of oscillatory profiles of canonical clock genes is not only useful for physiological and pathological examination of the circadian clock in various organs but also important for systematic understanding of transcriptional regulation on a genome-wide basis. Our finding of the oscillatory expression of canonical clock genes with a temporal order provides us an interesting hypothesis, that cyclic timing of all clock and clock-controlled genes may be dependent on several transcriptional elements including 3 known elements, E-box, RORE, and DBPE. BioMed Central 2004-10-09 /pmc/articles/PMC535906/ /pubmed/15473909 http://dx.doi.org/10.1186/1471-2199-5-18 Text en Copyright © 2004 Yamamoto et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yamamoto, Takuro Nakahata, Yasukazu Soma, Haruhiko Akashi, Makoto Mamine, Takayoshi Takumi, Toru Transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
title | Transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
title_full | Transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
title_fullStr | Transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
title_full_unstemmed | Transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
title_short | Transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
title_sort | transcriptional oscillation of canonical clock genes in mouse peripheral tissues |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC535906/ https://www.ncbi.nlm.nih.gov/pubmed/15473909 http://dx.doi.org/10.1186/1471-2199-5-18 |
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