A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification

A simple and non-radioactive technique based on O-cresolpthalein complexone assay was developed to study in vitro non-radioactive calcium ((40)Ca) deposition by isolated matrix vesicles. Using this technique, the effect of various phosphoester substrates including ATP, AMP and β-GP on in vitro MV-ca...

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Detalles Bibliográficos
Autores principales: Garimella, Rama, Sipe, Joseph B., Anderson, H. Clarke
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC535922/
https://www.ncbi.nlm.nih.gov/pubmed/15605107
http://dx.doi.org/10.1251/bpo97
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author Garimella, Rama
Sipe, Joseph B.
Anderson, H. Clarke
author_facet Garimella, Rama
Sipe, Joseph B.
Anderson, H. Clarke
author_sort Garimella, Rama
collection PubMed
description A simple and non-radioactive technique based on O-cresolpthalein complexone assay was developed to study in vitro non-radioactive calcium ((40)Ca) deposition by isolated matrix vesicles. Using this technique, the effect of various phosphoester substrates including ATP, AMP and β-GP on in vitro MV-calcification was studied. O-cresolpthalein complexone assay with non-radioactive calcium demonstrated that AMP or β-GP were more effective in promoting calcium deposition by isolated MVs than ATP. The application of this non-radioactive technique, which is highly sensitive and simple, would offer a useful alternative approach to the routinely used radiometric biomineralization assay which employs radioactive (45)Ca.
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spelling pubmed-5359222004-12-16 A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification Garimella, Rama Sipe, Joseph B. Anderson, H. Clarke Biol Proced Online Research Article A simple and non-radioactive technique based on O-cresolpthalein complexone assay was developed to study in vitro non-radioactive calcium ((40)Ca) deposition by isolated matrix vesicles. Using this technique, the effect of various phosphoester substrates including ATP, AMP and β-GP on in vitro MV-calcification was studied. O-cresolpthalein complexone assay with non-radioactive calcium demonstrated that AMP or β-GP were more effective in promoting calcium deposition by isolated MVs than ATP. The application of this non-radioactive technique, which is highly sensitive and simple, would offer a useful alternative approach to the routinely used radiometric biomineralization assay which employs radioactive (45)Ca. Biological Procedures Online 2004-12-16 /pmc/articles/PMC535922/ /pubmed/15605107 http://dx.doi.org/10.1251/bpo97 Text en Copyright © December 12, 2004, R Garimella et al. This paper is Open Access and is published in Biological Procedures Online under license from the authors. Copying, printing, redistribution and storage permitted.
spellingShingle Research Article
Garimella, Rama
Sipe, Joseph B.
Anderson, H. Clarke
A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
title A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
title_full A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
title_fullStr A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
title_full_unstemmed A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
title_short A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
title_sort simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC535922/
https://www.ncbi.nlm.nih.gov/pubmed/15605107
http://dx.doi.org/10.1251/bpo97
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