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Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis
This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359444/ https://www.ncbi.nlm.nih.gov/pubmed/28367456 http://dx.doi.org/10.1155/2017/5871043 |
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author | Link, Juliana Seger Alban, Silvana Maria Soccol, Carlos Ricardo Pereira, Gilberto Vinicius Melo Thomaz Soccol, Vanete |
author_facet | Link, Juliana Seger Alban, Silvana Maria Soccol, Carlos Ricardo Pereira, Gilberto Vinicius Melo Thomaz Soccol, Vanete |
author_sort | Link, Juliana Seger |
collection | PubMed |
description | This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund's adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L. braziliensis. The research on the similarity of the peptides' sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides' MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis. |
format | Online Article Text |
id | pubmed-5359444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-53594442017-04-02 Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis Link, Juliana Seger Alban, Silvana Maria Soccol, Carlos Ricardo Pereira, Gilberto Vinicius Melo Thomaz Soccol, Vanete J Immunol Res Research Article This work's goal was to research new candidate antigens for cutaneous leishmaniosis (CL). In order to reach the goal, we used random peptide phage display libraries screened using antibodies from Leishmania braziliensis patients. After selection, three peptides (P1, P2, and P3) were synthesized using Fmoc chemistry. The peptides individually or a mixture of them (MIX) was subsequently emulsified in complete and incomplete Freund's adjuvant and injected subcutaneously in golden hamsters. Sera from the hamsters administered with P1 presented antibodies that recognized proteins between 76 and 150 kDa from L. braziliensis. Sera from hamsters which had peptides P2 and P3, as well as the MIX, administered presented antibodies that recognized proteins between 52 and 76 kDa of L. braziliensis. The research on the similarity of the peptides' sequences in protein databases showed that they match a 63 kDa glycoprotein. The three peptides and the MIX were recognized by the sera from CL patients by immunoassay approach (ELISA). The peptides' MIX showed the best performance (79% sensitivity) followed by the P1 (72% sensitivity), and the AS presented 91% sensitivity. These results show a new route for discovering molecules for diagnosis or for immunoprotection against leishmaniosis. Hindawi 2017 2017-03-07 /pmc/articles/PMC5359444/ /pubmed/28367456 http://dx.doi.org/10.1155/2017/5871043 Text en Copyright © 2017 Juliana Seger Link et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Link, Juliana Seger Alban, Silvana Maria Soccol, Carlos Ricardo Pereira, Gilberto Vinicius Melo Thomaz Soccol, Vanete Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
title | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
title_full | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
title_fullStr | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
title_full_unstemmed | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
title_short | Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis |
title_sort | synthetic peptides as potential antigens for cutaneous leishmaniosis diagnosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359444/ https://www.ncbi.nlm.nih.gov/pubmed/28367456 http://dx.doi.org/10.1155/2017/5871043 |
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