5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila

5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the developmen...

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Autores principales: Huang, Yu Shan, Ku, Hui Yu, Tsai, Yun Chi, Chang, Chin Hao, Pao, Sih Hua, Sun, Y. Henry, Chiou, Arthur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359570/
https://www.ncbi.nlm.nih.gov/pubmed/28322328
http://dx.doi.org/10.1038/srep44945
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author Huang, Yu Shan
Ku, Hui Yu
Tsai, Yun Chi
Chang, Chin Hao
Pao, Sih Hua
Sun, Y. Henry
Chiou, Arthur
author_facet Huang, Yu Shan
Ku, Hui Yu
Tsai, Yun Chi
Chang, Chin Hao
Pao, Sih Hua
Sun, Y. Henry
Chiou, Arthur
author_sort Huang, Yu Shan
collection PubMed
description 5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en-expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en-expressing head segments.
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spelling pubmed-53595702017-03-22 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila Huang, Yu Shan Ku, Hui Yu Tsai, Yun Chi Chang, Chin Hao Pao, Sih Hua Sun, Y. Henry Chiou, Arthur Sci Rep Article 5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en-expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en-expressing head segments. Nature Publishing Group 2017-03-21 /pmc/articles/PMC5359570/ /pubmed/28322328 http://dx.doi.org/10.1038/srep44945 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Huang, Yu Shan
Ku, Hui Yu
Tsai, Yun Chi
Chang, Chin Hao
Pao, Sih Hua
Sun, Y. Henry
Chiou, Arthur
5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila
title 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila
title_full 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila
title_fullStr 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila
title_full_unstemmed 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila
title_short 5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila
title_sort 5d imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359570/
https://www.ncbi.nlm.nih.gov/pubmed/28322328
http://dx.doi.org/10.1038/srep44945
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