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Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum

Fusobacterium nucleatum plays a key role in creating the pathogenic subgingival biofilm that initiates destructive periodontitis. It is also a common resident of the human gastrointestinal tract and has been associated with inflammatory bowel disease. The aim of the present study was to investigate...

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Autores principales: Ben Lagha, Amel, Haas, Bruno, Grenier, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359671/
https://www.ncbi.nlm.nih.gov/pubmed/28322293
http://dx.doi.org/10.1038/srep44815
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author Ben Lagha, Amel
Haas, Bruno
Grenier, Daniel
author_facet Ben Lagha, Amel
Haas, Bruno
Grenier, Daniel
author_sort Ben Lagha, Amel
collection PubMed
description Fusobacterium nucleatum plays a key role in creating the pathogenic subgingival biofilm that initiates destructive periodontitis. It is also a common resident of the human gastrointestinal tract and has been associated with inflammatory bowel disease. The aim of the present study was to investigate the effects of green and black tea extracts as well as two of their bioactive components, EGCG and theaflavins, on the growth and virulence properties of F. nucleatum. The tea extracts and components displayed various degrees of antibacterial activity that may involve damage to the bacterial cell membrane and the chelation of iron. They also prevented biofilm formation by F. nucleatum at concentrations that did not interfere with bacterial growth. In addition, the treatment of a pre-formed F. nucleatum biofilm with the green tea extract and EGCG caused a time-dependent decrease in biofilm viability. The green and black tea extracts, EGCG, and theaflavins decreased the adherence of F. nucleatum to oral epithelial cells and matrix proteins. Moreover, these tea components also attenuated F. nucleatum-mediated hemolysis and hydrogen sulfide production, two other virulence factors expressed by this bacterium. In summary, this study showed that tea polyphenols may be of interest for treating F. nucleatum-associated disorders.
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spelling pubmed-53596712017-03-22 Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum Ben Lagha, Amel Haas, Bruno Grenier, Daniel Sci Rep Article Fusobacterium nucleatum plays a key role in creating the pathogenic subgingival biofilm that initiates destructive periodontitis. It is also a common resident of the human gastrointestinal tract and has been associated with inflammatory bowel disease. The aim of the present study was to investigate the effects of green and black tea extracts as well as two of their bioactive components, EGCG and theaflavins, on the growth and virulence properties of F. nucleatum. The tea extracts and components displayed various degrees of antibacterial activity that may involve damage to the bacterial cell membrane and the chelation of iron. They also prevented biofilm formation by F. nucleatum at concentrations that did not interfere with bacterial growth. In addition, the treatment of a pre-formed F. nucleatum biofilm with the green tea extract and EGCG caused a time-dependent decrease in biofilm viability. The green and black tea extracts, EGCG, and theaflavins decreased the adherence of F. nucleatum to oral epithelial cells and matrix proteins. Moreover, these tea components also attenuated F. nucleatum-mediated hemolysis and hydrogen sulfide production, two other virulence factors expressed by this bacterium. In summary, this study showed that tea polyphenols may be of interest for treating F. nucleatum-associated disorders. Nature Publishing Group 2017-03-21 /pmc/articles/PMC5359671/ /pubmed/28322293 http://dx.doi.org/10.1038/srep44815 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ben Lagha, Amel
Haas, Bruno
Grenier, Daniel
Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum
title Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum
title_full Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum
title_fullStr Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum
title_full_unstemmed Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum
title_short Tea polyphenols inhibit the growth and virulence properties of Fusobacterium nucleatum
title_sort tea polyphenols inhibit the growth and virulence properties of fusobacterium nucleatum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359671/
https://www.ncbi.nlm.nih.gov/pubmed/28322293
http://dx.doi.org/10.1038/srep44815
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