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Nelumbo nucifera leaves extracts inhibit mouse airway smooth muscle contraction

BACKGROUND: Alkaloids extracted from lotus leaves (AELL) can relax vascular smooth muscle. However, whether AELL has a similar relaxant role on airway smooth muscle (ASM) remains unknown. This study aimed to explore the relaxant property of AELL on ASM and the underlying mechanism. METHODS: Alkaloid...

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Detalles Bibliográficos
Autores principales: Yang, Xiao, Xue, Lu, Zhao, Qingyang, Cai, Congli, Liu, Qing-Hua, Shen, Jinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5359798/
https://www.ncbi.nlm.nih.gov/pubmed/28320373
http://dx.doi.org/10.1186/s12906-017-1674-7
Descripción
Sumario:BACKGROUND: Alkaloids extracted from lotus leaves (AELL) can relax vascular smooth muscle. However, whether AELL has a similar relaxant role on airway smooth muscle (ASM) remains unknown. This study aimed to explore the relaxant property of AELL on ASM and the underlying mechanism. METHODS: Alkaloids were extracted from dried lotus leaves using the high temperature rotary evaporation extraction method. The effects of AELL on mouse ASM tension were studied using force measuring and patch-clamp techniques. RESULTS: It was found that AELL inhibited the high K(+) or acetylcholine chloride (ACh)-induced precontraction of mouse tracheal rings by 64.8 ± 2.9%, or 48.8 ± 4.7%, respectively. The inhibition was statistically significant and performed in a dose-dependent manner. Furthermore, AELL-induced smooth muscle relaxation was partially mediated by blocking voltage-dependent Ca(2+) channels (VDCC) and non-selective cation channels (NSCC). CONCLUSION: AELL, which plays a relaxant role in ASM, might be a new complementary treatment to treat abnormal contractions of the trachea and asthma. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-017-1674-7) contains supplementary material, which is available to authorized users.