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Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand
Despite the strong association between Cryptococcus neoformans infection and the Human immunodeficiency virus (HIV) status of patients globally, most cryptococcosis cases in Far East Asia occur in non-HIV individuals. Molecular epidemiological studies, using multilocus sequence typing (MLST), have s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360237/ https://www.ncbi.nlm.nih.gov/pubmed/28323835 http://dx.doi.org/10.1371/journal.pone.0173744 |
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author | Hatthakaroon, Chanin Pharkjaksu, Sujiraphong Chongtrakool, Piriyaporn Suwannakarn, Kamol Kiratisin, Pattarachai Ngamskulrungroj, Popchai |
author_facet | Hatthakaroon, Chanin Pharkjaksu, Sujiraphong Chongtrakool, Piriyaporn Suwannakarn, Kamol Kiratisin, Pattarachai Ngamskulrungroj, Popchai |
author_sort | Hatthakaroon, Chanin |
collection | PubMed |
description | Despite the strong association between Cryptococcus neoformans infection and the Human immunodeficiency virus (HIV) status of patients globally, most cryptococcosis cases in Far East Asia occur in non-HIV individuals. Molecular epidemiological studies, using multilocus sequence typing (MLST), have shown that more than 95% of cryptococcal strains belong to a specific subtype of VNI. However, this association has never been specifically examined in other parts of Asia. Therefore, in this study, we investigated the VNIc/ST5 genotype distribution among cryptococcosis patients in Thailand. Fifty-one C. neoformans isolates were collected from clinical samples in Siriraj Hospital, Bangkok, Thailand. The strains were predominantly isolated from HIV-positive patients (88.57%) and all were molecular type VNI MATα. An MLST analysis identified five sequence types (ST) in Siriraj Hospital, of which ST4 (45.10%) and ST6 (35.29%) were most common, and ST5 (15.69%), ST32 (1.96%), and ST93 (1.96) were less common. Contrary to reports from Far East Asia, ST5 was predominantly (83.33%) found in HIV patients (P = 0.657), and there was no significant change in the prevalence of ST5 over the past 10 years (P = 0.548). A further analysis of comorbidities showed higher morbidity and delays in the cryptococcal diagnosis in patients with tuberculosis coinfection or without HIV. Our study suggests that although the Thai population is genetically closely related to the Far East Asian population, ST5 is not associated with non-HIV status in Thailand. Therefore, this association may not be related to the host’s genetic background. However, its mechanism remains unclear. |
format | Online Article Text |
id | pubmed-5360237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53602372017-04-06 Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand Hatthakaroon, Chanin Pharkjaksu, Sujiraphong Chongtrakool, Piriyaporn Suwannakarn, Kamol Kiratisin, Pattarachai Ngamskulrungroj, Popchai PLoS One Research Article Despite the strong association between Cryptococcus neoformans infection and the Human immunodeficiency virus (HIV) status of patients globally, most cryptococcosis cases in Far East Asia occur in non-HIV individuals. Molecular epidemiological studies, using multilocus sequence typing (MLST), have shown that more than 95% of cryptococcal strains belong to a specific subtype of VNI. However, this association has never been specifically examined in other parts of Asia. Therefore, in this study, we investigated the VNIc/ST5 genotype distribution among cryptococcosis patients in Thailand. Fifty-one C. neoformans isolates were collected from clinical samples in Siriraj Hospital, Bangkok, Thailand. The strains were predominantly isolated from HIV-positive patients (88.57%) and all were molecular type VNI MATα. An MLST analysis identified five sequence types (ST) in Siriraj Hospital, of which ST4 (45.10%) and ST6 (35.29%) were most common, and ST5 (15.69%), ST32 (1.96%), and ST93 (1.96) were less common. Contrary to reports from Far East Asia, ST5 was predominantly (83.33%) found in HIV patients (P = 0.657), and there was no significant change in the prevalence of ST5 over the past 10 years (P = 0.548). A further analysis of comorbidities showed higher morbidity and delays in the cryptococcal diagnosis in patients with tuberculosis coinfection or without HIV. Our study suggests that although the Thai population is genetically closely related to the Far East Asian population, ST5 is not associated with non-HIV status in Thailand. Therefore, this association may not be related to the host’s genetic background. However, its mechanism remains unclear. Public Library of Science 2017-03-21 /pmc/articles/PMC5360237/ /pubmed/28323835 http://dx.doi.org/10.1371/journal.pone.0173744 Text en © 2017 Hatthakaroon et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hatthakaroon, Chanin Pharkjaksu, Sujiraphong Chongtrakool, Piriyaporn Suwannakarn, Kamol Kiratisin, Pattarachai Ngamskulrungroj, Popchai Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand |
title | Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand |
title_full | Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand |
title_fullStr | Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand |
title_full_unstemmed | Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand |
title_short | Molecular epidemiology of cryptococcal genotype VNIc/ST5 in Siriraj Hospital, Thailand |
title_sort | molecular epidemiology of cryptococcal genotype vnic/st5 in siriraj hospital, thailand |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360237/ https://www.ncbi.nlm.nih.gov/pubmed/28323835 http://dx.doi.org/10.1371/journal.pone.0173744 |
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