Cargando…

Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer

Transmembrane protease serine 4 (TMPRSS4) is a novel type II transmembrane serine protease that is overexpressed in various types of human cancers and has an important function in cancer progression. However, there is a paucity of data available regarding the biological effects of TMPRSS4 on breast...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiao-Mei, Liu, Wen-Lou, Chen, Xu, Wang, Ya-Wen, Shi, Duan-Bo, Zhang, Hui, Ma, Ran-Ran, Liu, Hai-Ting, Guo, Xiang-Yu, Hou, Feng, Li, Ming, Gao, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360421/
https://www.ncbi.nlm.nih.gov/pubmed/28259959
http://dx.doi.org/10.3892/ijmm.2017.2893
_version_ 1782516599205396480
author Li, Xiao-Mei
Liu, Wen-Lou
Chen, Xu
Wang, Ya-Wen
Shi, Duan-Bo
Zhang, Hui
Ma, Ran-Ran
Liu, Hai-Ting
Guo, Xiang-Yu
Hou, Feng
Li, Ming
Gao, Peng
author_facet Li, Xiao-Mei
Liu, Wen-Lou
Chen, Xu
Wang, Ya-Wen
Shi, Duan-Bo
Zhang, Hui
Ma, Ran-Ran
Liu, Hai-Ting
Guo, Xiang-Yu
Hou, Feng
Li, Ming
Gao, Peng
author_sort Li, Xiao-Mei
collection PubMed
description Transmembrane protease serine 4 (TMPRSS4) is a novel type II transmembrane serine protease that is overexpressed in various types of human cancers and has an important function in cancer progression. However, there is a paucity of data available regarding the biological effects of TMPRSS4 on breast cancer (BC) cells and the underlying mechanisms. In this study, expression of TMPRSS4 in BC tissues was detected by immunohistochemistry. The relationship between TMPRSS4 expression and clinicopathological characteristics as well as prognosis was evaluated. The effects of TMPRSS4 on cell proliferation, migration and invasion were investigated in BC cell lines in vitro. Additionally, RT-qPCR and western blot analysis were used to determine the expressions of epithelial-mesenchymal transition (EMT) biomarkers and TMPRSS4 in BC cell lines. We found that TMPRSS4 was overexpressed in BC tissues and its expression level was closely correlated with tumor size, histological grade, lymph node metastasis, clinical stage as well as poor survival (all P<0.05) and could be recognized as an independent prognostic factor for BC patients. Overexpression of TMPRSS4 promoted the proliferation, migration and invasion of BC cells in vitro. Moreover, TMPRSS4 knockdown significantly enhanced the expression of E-cadherin and claudin-1 and inhibited the expression of vimentin and Slug, indicating suppression of EMT. Our results suggest that TMPRSS4 plays a crucial role in the progression of BC. Moreover, TMPRSS4 overexpression promoted the proliferation, invasion and migration of BC cells by possibly inducing EMT. To conclude, TMPRSS4 may be a potential therapeutic target for cancer treatment.
format Online
Article
Text
id pubmed-5360421
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-53604212017-04-10 Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer Li, Xiao-Mei Liu, Wen-Lou Chen, Xu Wang, Ya-Wen Shi, Duan-Bo Zhang, Hui Ma, Ran-Ran Liu, Hai-Ting Guo, Xiang-Yu Hou, Feng Li, Ming Gao, Peng Int J Mol Med Articles Transmembrane protease serine 4 (TMPRSS4) is a novel type II transmembrane serine protease that is overexpressed in various types of human cancers and has an important function in cancer progression. However, there is a paucity of data available regarding the biological effects of TMPRSS4 on breast cancer (BC) cells and the underlying mechanisms. In this study, expression of TMPRSS4 in BC tissues was detected by immunohistochemistry. The relationship between TMPRSS4 expression and clinicopathological characteristics as well as prognosis was evaluated. The effects of TMPRSS4 on cell proliferation, migration and invasion were investigated in BC cell lines in vitro. Additionally, RT-qPCR and western blot analysis were used to determine the expressions of epithelial-mesenchymal transition (EMT) biomarkers and TMPRSS4 in BC cell lines. We found that TMPRSS4 was overexpressed in BC tissues and its expression level was closely correlated with tumor size, histological grade, lymph node metastasis, clinical stage as well as poor survival (all P<0.05) and could be recognized as an independent prognostic factor for BC patients. Overexpression of TMPRSS4 promoted the proliferation, migration and invasion of BC cells in vitro. Moreover, TMPRSS4 knockdown significantly enhanced the expression of E-cadherin and claudin-1 and inhibited the expression of vimentin and Slug, indicating suppression of EMT. Our results suggest that TMPRSS4 plays a crucial role in the progression of BC. Moreover, TMPRSS4 overexpression promoted the proliferation, invasion and migration of BC cells by possibly inducing EMT. To conclude, TMPRSS4 may be a potential therapeutic target for cancer treatment. D.A. Spandidos 2017-04 2017-02-17 /pmc/articles/PMC5360421/ /pubmed/28259959 http://dx.doi.org/10.3892/ijmm.2017.2893 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Xiao-Mei
Liu, Wen-Lou
Chen, Xu
Wang, Ya-Wen
Shi, Duan-Bo
Zhang, Hui
Ma, Ran-Ran
Liu, Hai-Ting
Guo, Xiang-Yu
Hou, Feng
Li, Ming
Gao, Peng
Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer
title Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer
title_full Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer
title_fullStr Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer
title_full_unstemmed Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer
title_short Overexpression of TMPRSS4 promotes tumor proliferation and aggressiveness in breast cancer
title_sort overexpression of tmprss4 promotes tumor proliferation and aggressiveness in breast cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360421/
https://www.ncbi.nlm.nih.gov/pubmed/28259959
http://dx.doi.org/10.3892/ijmm.2017.2893
work_keys_str_mv AT lixiaomei overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT liuwenlou overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT chenxu overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT wangyawen overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT shiduanbo overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT zhanghui overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT maranran overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT liuhaiting overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT guoxiangyu overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT houfeng overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT liming overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer
AT gaopeng overexpressionoftmprss4promotestumorproliferationandaggressivenessinbreastcancer