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An ongoing role for Wnt signaling in differentiating melanocytes in vivo
A role for Wnt signaling in melanocyte specification from neural crest is conserved across vertebrates, but possible ongoing roles in melanocyte differentiation have received little attention. Using a systems biology approach to investigate the gene regulatory network underlying stable melanocyte di...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360516/ https://www.ncbi.nlm.nih.gov/pubmed/27977907 http://dx.doi.org/10.1111/pcmr.12568 |
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author | Vibert, Laura Aquino, Gerardo Gehring, Ines Subkankulova, Tatiana Schilling, Thomas F. Rocco, Andrea Kelsh, Robert N. |
author_facet | Vibert, Laura Aquino, Gerardo Gehring, Ines Subkankulova, Tatiana Schilling, Thomas F. Rocco, Andrea Kelsh, Robert N. |
author_sort | Vibert, Laura |
collection | PubMed |
description | A role for Wnt signaling in melanocyte specification from neural crest is conserved across vertebrates, but possible ongoing roles in melanocyte differentiation have received little attention. Using a systems biology approach to investigate the gene regulatory network underlying stable melanocyte differentiation in zebrafish highlighted a requirement for a positive‐feedback loop involving the melanocyte master regulator Mitfa. Here, we test the hypothesis that Wnt signaling contributes to that positive feedback. We show firstly that Wnt signaling remains active in differentiating melanocytes and secondly that enhanced Wnt signaling drives elevated transcription of mitfa. We show that chemical activation of the Wnt signaling pathway at early stages of melanocyte development enhances melanocyte specification as expected, but importantly that at later (differentiation) stages, it results in altered melanocyte morphology, although melanisation is not obviously affected. Downregulation of Wnt signaling also results in altered melanocyte morphology and organization. We conclude that Wnt signaling plays a role in regulating ongoing aspects of melanocyte differentiation in zebrafish. |
format | Online Article Text |
id | pubmed-5360516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53605162017-05-04 An ongoing role for Wnt signaling in differentiating melanocytes in vivo Vibert, Laura Aquino, Gerardo Gehring, Ines Subkankulova, Tatiana Schilling, Thomas F. Rocco, Andrea Kelsh, Robert N. Pigment Cell Melanoma Res Original Articles A role for Wnt signaling in melanocyte specification from neural crest is conserved across vertebrates, but possible ongoing roles in melanocyte differentiation have received little attention. Using a systems biology approach to investigate the gene regulatory network underlying stable melanocyte differentiation in zebrafish highlighted a requirement for a positive‐feedback loop involving the melanocyte master regulator Mitfa. Here, we test the hypothesis that Wnt signaling contributes to that positive feedback. We show firstly that Wnt signaling remains active in differentiating melanocytes and secondly that enhanced Wnt signaling drives elevated transcription of mitfa. We show that chemical activation of the Wnt signaling pathway at early stages of melanocyte development enhances melanocyte specification as expected, but importantly that at later (differentiation) stages, it results in altered melanocyte morphology, although melanisation is not obviously affected. Downregulation of Wnt signaling also results in altered melanocyte morphology and organization. We conclude that Wnt signaling plays a role in regulating ongoing aspects of melanocyte differentiation in zebrafish. John Wiley and Sons Inc. 2017-03-09 2017-03 /pmc/articles/PMC5360516/ /pubmed/27977907 http://dx.doi.org/10.1111/pcmr.12568 Text en © 2016 The Authors. Pigment Cell & Melanoma Research Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Vibert, Laura Aquino, Gerardo Gehring, Ines Subkankulova, Tatiana Schilling, Thomas F. Rocco, Andrea Kelsh, Robert N. An ongoing role for Wnt signaling in differentiating melanocytes in vivo |
title | An ongoing role for Wnt signaling in differentiating melanocytes in vivo |
title_full | An ongoing role for Wnt signaling in differentiating melanocytes in vivo |
title_fullStr | An ongoing role for Wnt signaling in differentiating melanocytes in vivo |
title_full_unstemmed | An ongoing role for Wnt signaling in differentiating melanocytes in vivo |
title_short | An ongoing role for Wnt signaling in differentiating melanocytes in vivo |
title_sort | ongoing role for wnt signaling in differentiating melanocytes in vivo |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360516/ https://www.ncbi.nlm.nih.gov/pubmed/27977907 http://dx.doi.org/10.1111/pcmr.12568 |
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