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Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia

To examine the mechanism of ocular axial elongation in myopia, guinea pigs (age: 2–3 weeks) which either underwent unilateral or bilateral lens-induced myopization (group 1) or which were primarily myopic at baseline (group 2) received unilateral intraocular injections of amphiregulin antibody (dose...

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Autores principales: Jiang, Wen Jun, Song, Hui Xin, Li, Shao Yu, Guo, Bin, Wu, Jian Feng, Li, Guo Ping, Guo, Da Dong, Shi, De Long, Bi, Hong Sheng, Jonas, Jost B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360597/
https://www.ncbi.nlm.nih.gov/pubmed/28256400
http://dx.doi.org/10.1016/j.ebiom.2017.02.021
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author Jiang, Wen Jun
Song, Hui Xin
Li, Shao Yu
Guo, Bin
Wu, Jian Feng
Li, Guo Ping
Guo, Da Dong
Shi, De Long
Bi, Hong Sheng
Jonas, Jost B.
author_facet Jiang, Wen Jun
Song, Hui Xin
Li, Shao Yu
Guo, Bin
Wu, Jian Feng
Li, Guo Ping
Guo, Da Dong
Shi, De Long
Bi, Hong Sheng
Jonas, Jost B.
author_sort Jiang, Wen Jun
collection PubMed
description To examine the mechanism of ocular axial elongation in myopia, guinea pigs (age: 2–3 weeks) which either underwent unilateral or bilateral lens-induced myopization (group 1) or which were primarily myopic at baseline (group 2) received unilateral intraocular injections of amphiregulin antibody (doses: 5, 10, or 15 μg) three times in intervals of 9 days. A third group of emmetropic guinea pigs got intraocular unilateral injections of amphiregulin (doses: 0.25, 0.50 or 1.00 ng, respectively). In each group, the contralateral eyes received intraocular injections of Ringer's solution. In intra-animal inter-eye comparison and intra-eye follow-up comparison in groups 1 and 2, the study eyes as compared to the contralateral eyes showed a dose-dependent reduction in axial elongation. In group 3, study eyes and control eyes did not differ significantly in axial elongation. Immunohistochemistry revealed amphiregulin labelling at the retinal pigment epithelium in eyes with lens-induced myopization and Ringer's solution injection, but not in eyes with amphiregulin antibody injection. Intraocular injections of amphiregulin-antibody led to a reduction of lens-induced axial myopic elongation and of the physiological eye enlargement in young guinea pigs. In contrast, intraocularly injected amphiregulin in a dose of ≤ 1 ng did not show a significant effect. Amphiregulin may be one of several essential molecular factors for axial elongation.
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spelling pubmed-53605972017-03-30 Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia Jiang, Wen Jun Song, Hui Xin Li, Shao Yu Guo, Bin Wu, Jian Feng Li, Guo Ping Guo, Da Dong Shi, De Long Bi, Hong Sheng Jonas, Jost B. EBioMedicine Research Paper To examine the mechanism of ocular axial elongation in myopia, guinea pigs (age: 2–3 weeks) which either underwent unilateral or bilateral lens-induced myopization (group 1) or which were primarily myopic at baseline (group 2) received unilateral intraocular injections of amphiregulin antibody (doses: 5, 10, or 15 μg) three times in intervals of 9 days. A third group of emmetropic guinea pigs got intraocular unilateral injections of amphiregulin (doses: 0.25, 0.50 or 1.00 ng, respectively). In each group, the contralateral eyes received intraocular injections of Ringer's solution. In intra-animal inter-eye comparison and intra-eye follow-up comparison in groups 1 and 2, the study eyes as compared to the contralateral eyes showed a dose-dependent reduction in axial elongation. In group 3, study eyes and control eyes did not differ significantly in axial elongation. Immunohistochemistry revealed amphiregulin labelling at the retinal pigment epithelium in eyes with lens-induced myopization and Ringer's solution injection, but not in eyes with amphiregulin antibody injection. Intraocular injections of amphiregulin-antibody led to a reduction of lens-induced axial myopic elongation and of the physiological eye enlargement in young guinea pigs. In contrast, intraocularly injected amphiregulin in a dose of ≤ 1 ng did not show a significant effect. Amphiregulin may be one of several essential molecular factors for axial elongation. Elsevier 2017-02-23 /pmc/articles/PMC5360597/ /pubmed/28256400 http://dx.doi.org/10.1016/j.ebiom.2017.02.021 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Jiang, Wen Jun
Song, Hui Xin
Li, Shao Yu
Guo, Bin
Wu, Jian Feng
Li, Guo Ping
Guo, Da Dong
Shi, De Long
Bi, Hong Sheng
Jonas, Jost B.
Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
title Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
title_full Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
title_fullStr Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
title_full_unstemmed Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
title_short Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
title_sort amphiregulin antibody and reduction of axial elongation in experimental myopia
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360597/
https://www.ncbi.nlm.nih.gov/pubmed/28256400
http://dx.doi.org/10.1016/j.ebiom.2017.02.021
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