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Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize
Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be used in monocots, by presenting a system involving use of a novel infiltration solution (containing acetosyringone, cysteine, and Tween 20) that enables whole-...
Autores principales: | Zhang, Ju, Yu, Deshui, Zhang, Yi, Liu, Kun, Xu, Kedong, Zhang, Fuli, Wang, Jian, Tan, Guangxuan, Nie, Xianhui, Ji, Qiaohua, Zhao, Lu, Li, Chengwei |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360694/ https://www.ncbi.nlm.nih.gov/pubmed/28382049 http://dx.doi.org/10.3389/fpls.2017.00393 |
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