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Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications
Recombinant chimeric α-amylase (Ba-Gt-amy) has been produced extracellularly in Pichia pastoris under AOX promoter. Clones of P. pastoris with multiple gene copies have been generated by multiple transformations and post-transformational vector amplification, which led to 10.7-fold enhancement in α-...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Frontiers Media S.A.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360700/ https://www.ncbi.nlm.nih.gov/pubmed/28382032 http://dx.doi.org/10.3389/fmicb.2017.00493 |