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MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development
The role of microRNAs (miRNAs) during cotton fiber development remains unclear. Here, a total of 54 miRNAs belonging to 39 families were selected to characterize miRNA regulatory mechanism in eight different fiber development stages in upland cotton cv BM-1. Among 54 miRNAs, 18 miRNAs were involved...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361117/ https://www.ncbi.nlm.nih.gov/pubmed/28327647 http://dx.doi.org/10.1038/srep44454 |
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author | Wang, Min Sun, Runrun Li, Chao Wang, Qinglian Zhang, Baohong |
author_facet | Wang, Min Sun, Runrun Li, Chao Wang, Qinglian Zhang, Baohong |
author_sort | Wang, Min |
collection | PubMed |
description | The role of microRNAs (miRNAs) during cotton fiber development remains unclear. Here, a total of 54 miRNAs belonging to 39 families were selected to characterize miRNA regulatory mechanism in eight different fiber development stages in upland cotton cv BM-1. Among 54 miRNAs, 18 miRNAs were involved in cotton fiber initiation and eight miRNAs were related to fiber elongation and secondary wall biosynthesis. Additionally, 3,576 protein-coding genes were candidate target genes of these miRNAs, which are potentially involved in cotton fiber development. We also investigated the regulatory network of miRNAs and corresponding targets in fiber initiation and elongation, and secondary wall formation. Our Gene Ontology-based term classification and KEGG-based pathway enrichment analyses showed that the miRNA targets covered 220 biological processes, 67 molecular functions, 45 cellular components, and 10 KEGG pathways. Three of ten KEGG pathways were involved in lignan synthesis, cell elongation, and fatty acid biosynthesis, all of which have important roles in fiber development. Overall, our study shows the potential regulatory roles of miRNAs in cotton fiber development and the importance of miRNAs in regulating different cell types. This is helpful to design miRNA-based biotechnology for improving fiber quality and yield. |
format | Online Article Text |
id | pubmed-5361117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53611172017-03-24 MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development Wang, Min Sun, Runrun Li, Chao Wang, Qinglian Zhang, Baohong Sci Rep Article The role of microRNAs (miRNAs) during cotton fiber development remains unclear. Here, a total of 54 miRNAs belonging to 39 families were selected to characterize miRNA regulatory mechanism in eight different fiber development stages in upland cotton cv BM-1. Among 54 miRNAs, 18 miRNAs were involved in cotton fiber initiation and eight miRNAs were related to fiber elongation and secondary wall biosynthesis. Additionally, 3,576 protein-coding genes were candidate target genes of these miRNAs, which are potentially involved in cotton fiber development. We also investigated the regulatory network of miRNAs and corresponding targets in fiber initiation and elongation, and secondary wall formation. Our Gene Ontology-based term classification and KEGG-based pathway enrichment analyses showed that the miRNA targets covered 220 biological processes, 67 molecular functions, 45 cellular components, and 10 KEGG pathways. Three of ten KEGG pathways were involved in lignan synthesis, cell elongation, and fatty acid biosynthesis, all of which have important roles in fiber development. Overall, our study shows the potential regulatory roles of miRNAs in cotton fiber development and the importance of miRNAs in regulating different cell types. This is helpful to design miRNA-based biotechnology for improving fiber quality and yield. Nature Publishing Group 2017-03-22 /pmc/articles/PMC5361117/ /pubmed/28327647 http://dx.doi.org/10.1038/srep44454 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Min Sun, Runrun Li, Chao Wang, Qinglian Zhang, Baohong MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development |
title | MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development |
title_full | MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development |
title_fullStr | MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development |
title_full_unstemmed | MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development |
title_short | MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development |
title_sort | microrna expression profiles during cotton (gossypium hirsutum l) fiber early development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361117/ https://www.ncbi.nlm.nih.gov/pubmed/28327647 http://dx.doi.org/10.1038/srep44454 |
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