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Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors

The activation of adenosine A1 receptors is important for protecting against ischemic brain injury and pretreatment with electroacupuncture has been shown to mitigate ischemic brain insult. The aim of this study was to test whether the adenosine A1 receptor mediates electroacupuncture pretreatment-i...

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Autores principales: Dai, Qin-xue, Geng, Wu-jun, Zhuang, Xiu-xiu, Wang, Hong-fa, Mo, Yun-chang, Xin, He, Chen, Jiang-fan, Wang, Jun-lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361506/
https://www.ncbi.nlm.nih.gov/pubmed/28400804
http://dx.doi.org/10.4103/1673-5374.200806
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author Dai, Qin-xue
Geng, Wu-jun
Zhuang, Xiu-xiu
Wang, Hong-fa
Mo, Yun-chang
Xin, He
Chen, Jiang-fan
Wang, Jun-lu
author_facet Dai, Qin-xue
Geng, Wu-jun
Zhuang, Xiu-xiu
Wang, Hong-fa
Mo, Yun-chang
Xin, He
Chen, Jiang-fan
Wang, Jun-lu
author_sort Dai, Qin-xue
collection PubMed
description The activation of adenosine A1 receptors is important for protecting against ischemic brain injury and pretreatment with electroacupuncture has been shown to mitigate ischemic brain insult. The aim of this study was to test whether the adenosine A1 receptor mediates electroacupuncture pretreatment-induced neuroprotection against ischemic brain injury. We first performed 30 minutes of electroacupuncture pretreatment at the Baihui acupoint (GV20), delivered with a current of 1 mA, a frequency of 2/15 Hz, and a depth of 1 mm. High-performance liquid chromatography found that adenosine triphosphate and adenosine levels peaked in the cerebral cortex at 15 minutes and 120 minutes after electroacupuncture pretreatment, respectively. We further examined the effect of 15 or 120 minutes electroacupuncture treatment on ischemic brain injury in a rat middle cerebral artery-occlusion model. We found that at 24 hours reperfusion,120 minutes after electroacupuncture pretreatment, but not for 15 minutes, significantly reduced behavioral deficits and infarct volumes. Last, we demonstrated that the protective effect gained by 120 minutes after electroacupuncture treatment before ischemic injury was abolished by pretreatment with the A1-receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (1 mg/kg, intraperitoneally). Our results suggest that pretreatment with electroacupuncture at the Baihui acupoint elicits protection against transient cerebral ischemia via action at adenosine A1 receptors.
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spelling pubmed-53615062017-04-11 Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors Dai, Qin-xue Geng, Wu-jun Zhuang, Xiu-xiu Wang, Hong-fa Mo, Yun-chang Xin, He Chen, Jiang-fan Wang, Jun-lu Neural Regen Res Research Article The activation of adenosine A1 receptors is important for protecting against ischemic brain injury and pretreatment with electroacupuncture has been shown to mitigate ischemic brain insult. The aim of this study was to test whether the adenosine A1 receptor mediates electroacupuncture pretreatment-induced neuroprotection against ischemic brain injury. We first performed 30 minutes of electroacupuncture pretreatment at the Baihui acupoint (GV20), delivered with a current of 1 mA, a frequency of 2/15 Hz, and a depth of 1 mm. High-performance liquid chromatography found that adenosine triphosphate and adenosine levels peaked in the cerebral cortex at 15 minutes and 120 minutes after electroacupuncture pretreatment, respectively. We further examined the effect of 15 or 120 minutes electroacupuncture treatment on ischemic brain injury in a rat middle cerebral artery-occlusion model. We found that at 24 hours reperfusion,120 minutes after electroacupuncture pretreatment, but not for 15 minutes, significantly reduced behavioral deficits and infarct volumes. Last, we demonstrated that the protective effect gained by 120 minutes after electroacupuncture treatment before ischemic injury was abolished by pretreatment with the A1-receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (1 mg/kg, intraperitoneally). Our results suggest that pretreatment with electroacupuncture at the Baihui acupoint elicits protection against transient cerebral ischemia via action at adenosine A1 receptors. Medknow Publications & Media Pvt Ltd 2017-02 /pmc/articles/PMC5361506/ /pubmed/28400804 http://dx.doi.org/10.4103/1673-5374.200806 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Dai, Qin-xue
Geng, Wu-jun
Zhuang, Xiu-xiu
Wang, Hong-fa
Mo, Yun-chang
Xin, He
Chen, Jiang-fan
Wang, Jun-lu
Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
title Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
title_full Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
title_fullStr Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
title_full_unstemmed Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
title_short Electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine A1 receptors
title_sort electroacupuncture-induced neuroprotection against focal cerebral ischemia in the rat is mediated by adenosine a1 receptors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361506/
https://www.ncbi.nlm.nih.gov/pubmed/28400804
http://dx.doi.org/10.4103/1673-5374.200806
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