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Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites
Horizontal gene transfer has emerged as a crucial driving force for the evolution of eukaryotes. This also includes Plasmodium falciparum and related economically and clinically relevant apicomplexan parasites, whose rather small genomes have been shaped not only by natural selection in different ho...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Shared Science Publishers OG
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361646/ https://www.ncbi.nlm.nih.gov/pubmed/28357258 http://dx.doi.org/10.15698/mic2015.01.182 |
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author | Djuika, Carine F. Huerta-Cepas, Jaime Przyborski, Jude M. Deil, Sophia Sanchez, Cecilia P. Doerks, Tobias Bork, Peer Lanzer, Michael Deponte, Marcel |
author_facet | Djuika, Carine F. Huerta-Cepas, Jaime Przyborski, Jude M. Deil, Sophia Sanchez, Cecilia P. Doerks, Tobias Bork, Peer Lanzer, Michael Deponte, Marcel |
author_sort | Djuika, Carine F. |
collection | PubMed |
description | Horizontal gene transfer has emerged as a crucial driving force for the evolution of eukaryotes. This also includes Plasmodium falciparum and related economically and clinically relevant apicomplexan parasites, whose rather small genomes have been shaped not only by natural selection in different host populations but also by horizontal gene transfer following endosymbiosis. However, there is rather little reliable data on horizontal gene transfer between animal hosts or bacteria and apicomplexan parasites. Here we show that apicomplexan homologues of peroxiredoxin 5 (Prx5) have a prokaryotic ancestry and therefore represent a special subclass of Prx5 isoforms in eukaryotes. Using two different immunobiochemical approaches, we found that the P. falciparum Prx5 homologue is dually localized to the parasite plastid and cytosol. This dual localization is reflected by a modular Plasmodium-specific gene architecture consisting of two exons. Despite the plastid localization, our phylogenetic analyses contradict an acquisition by secondary endosymbiosis and support a gene fusion event following a horizontal prokaryote-to-eukaryote gene transfer in early apicomplexans. The results provide unexpected insights into the evolution of apicomplexan parasites as well as the molecular evolution of peroxiredoxins, an important family of ubiquitous, usually highly concentrated thiol-dependent hydroperoxidases that exert functions as detoxifying enzymes, redox sensors and chaperones. |
format | Online Article Text |
id | pubmed-5361646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Shared Science Publishers OG |
record_format | MEDLINE/PubMed |
spelling | pubmed-53616462017-03-29 Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites Djuika, Carine F. Huerta-Cepas, Jaime Przyborski, Jude M. Deil, Sophia Sanchez, Cecilia P. Doerks, Tobias Bork, Peer Lanzer, Michael Deponte, Marcel Microb Cell Microbiology Horizontal gene transfer has emerged as a crucial driving force for the evolution of eukaryotes. This also includes Plasmodium falciparum and related economically and clinically relevant apicomplexan parasites, whose rather small genomes have been shaped not only by natural selection in different host populations but also by horizontal gene transfer following endosymbiosis. However, there is rather little reliable data on horizontal gene transfer between animal hosts or bacteria and apicomplexan parasites. Here we show that apicomplexan homologues of peroxiredoxin 5 (Prx5) have a prokaryotic ancestry and therefore represent a special subclass of Prx5 isoforms in eukaryotes. Using two different immunobiochemical approaches, we found that the P. falciparum Prx5 homologue is dually localized to the parasite plastid and cytosol. This dual localization is reflected by a modular Plasmodium-specific gene architecture consisting of two exons. Despite the plastid localization, our phylogenetic analyses contradict an acquisition by secondary endosymbiosis and support a gene fusion event following a horizontal prokaryote-to-eukaryote gene transfer in early apicomplexans. The results provide unexpected insights into the evolution of apicomplexan parasites as well as the molecular evolution of peroxiredoxins, an important family of ubiquitous, usually highly concentrated thiol-dependent hydroperoxidases that exert functions as detoxifying enzymes, redox sensors and chaperones. Shared Science Publishers OG 2015-01-05 /pmc/articles/PMC5361646/ /pubmed/28357258 http://dx.doi.org/10.15698/mic2015.01.182 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged. |
spellingShingle | Microbiology Djuika, Carine F. Huerta-Cepas, Jaime Przyborski, Jude M. Deil, Sophia Sanchez, Cecilia P. Doerks, Tobias Bork, Peer Lanzer, Michael Deponte, Marcel Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
title | Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
title_full | Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
title_fullStr | Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
title_full_unstemmed | Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
title_short | Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
title_sort | prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malaria parasites |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361646/ https://www.ncbi.nlm.nih.gov/pubmed/28357258 http://dx.doi.org/10.15698/mic2015.01.182 |
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