Cargando…

Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects

Aging is associated with a decline in autophagy and a state of low-grade inflammation which further affects apoptosis and autophagy. Importantly, these alterations could reverse with regular physical activity. This study assessed the effects of a resistance exercise training program on autophagy, NL...

Descripción completa

Detalles Bibliográficos
Autores principales: Mejías-Peña, Yubisay, Estébanez, Brisamar, Rodriguez-Miguelez, Paula, Fernandez-Gonzalo, Rodrigo, Almar, Mar, de Paz, José A., González-Gallego, Javier, Cuevas, María J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361672/
https://www.ncbi.nlm.nih.gov/pubmed/28160545
http://dx.doi.org/10.18632/aging.101167
_version_ 1782516811366924288
author Mejías-Peña, Yubisay
Estébanez, Brisamar
Rodriguez-Miguelez, Paula
Fernandez-Gonzalo, Rodrigo
Almar, Mar
de Paz, José A.
González-Gallego, Javier
Cuevas, María J.
author_facet Mejías-Peña, Yubisay
Estébanez, Brisamar
Rodriguez-Miguelez, Paula
Fernandez-Gonzalo, Rodrigo
Almar, Mar
de Paz, José A.
González-Gallego, Javier
Cuevas, María J.
author_sort Mejías-Peña, Yubisay
collection PubMed
description Aging is associated with a decline in autophagy and a state of low-grade inflammation which further affects apoptosis and autophagy. Importantly, these alterations could reverse with regular physical activity. This study assessed the effects of a resistance exercise training program on autophagy, NLRP3 inflammasome, and apoptosis in peripheral blood mononuclear cells (PBMCs) from old subjects. Twenty-six healthy women and men (age, 69.6±1.5 yr) were randomized to a training (TG) or a control (CG) group. TG performed an 8-week resistance training program, while CG followed their daily routines. Protein expression of beclin-1, Atg12, Atg16 and LAMP-2 increased following the training program, while expression of p62/SQSTM1 and phosphorylation of ULK-1 at Ser757 were significantly lower. Resistance exercise also induced a decrease in NLRP3 expression and in the caspase-1/procaspase-1 ratio. Expression of Bcl-2 and Bcl-xL, as well as the Bad/BcL-2 ratio were reduced, and there was a significant decrease in the protein content of caspase-3. The results obtained seem to indicate that 8-week resistance training stimulates autophagy, prevents NLRP3 inflammasome activation, and reduces apoptosis in PBMCs from elderly subjects. These data could have a significant impact in prevention and rehabilitation programs currently employed in elderly population.
format Online
Article
Text
id pubmed-5361672
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-53616722017-03-29 Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects Mejías-Peña, Yubisay Estébanez, Brisamar Rodriguez-Miguelez, Paula Fernandez-Gonzalo, Rodrigo Almar, Mar de Paz, José A. González-Gallego, Javier Cuevas, María J. Aging (Albany NY) Research Paper Aging is associated with a decline in autophagy and a state of low-grade inflammation which further affects apoptosis and autophagy. Importantly, these alterations could reverse with regular physical activity. This study assessed the effects of a resistance exercise training program on autophagy, NLRP3 inflammasome, and apoptosis in peripheral blood mononuclear cells (PBMCs) from old subjects. Twenty-six healthy women and men (age, 69.6±1.5 yr) were randomized to a training (TG) or a control (CG) group. TG performed an 8-week resistance training program, while CG followed their daily routines. Protein expression of beclin-1, Atg12, Atg16 and LAMP-2 increased following the training program, while expression of p62/SQSTM1 and phosphorylation of ULK-1 at Ser757 were significantly lower. Resistance exercise also induced a decrease in NLRP3 expression and in the caspase-1/procaspase-1 ratio. Expression of Bcl-2 and Bcl-xL, as well as the Bad/BcL-2 ratio were reduced, and there was a significant decrease in the protein content of caspase-3. The results obtained seem to indicate that 8-week resistance training stimulates autophagy, prevents NLRP3 inflammasome activation, and reduces apoptosis in PBMCs from elderly subjects. These data could have a significant impact in prevention and rehabilitation programs currently employed in elderly population. Impact Journals LLC 2017-02-02 /pmc/articles/PMC5361672/ /pubmed/28160545 http://dx.doi.org/10.18632/aging.101167 Text en Copyright: © 2017 Mejías-Peña et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Mejías-Peña, Yubisay
Estébanez, Brisamar
Rodriguez-Miguelez, Paula
Fernandez-Gonzalo, Rodrigo
Almar, Mar
de Paz, José A.
González-Gallego, Javier
Cuevas, María J.
Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
title Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
title_full Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
title_fullStr Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
title_full_unstemmed Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
title_short Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
title_sort impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361672/
https://www.ncbi.nlm.nih.gov/pubmed/28160545
http://dx.doi.org/10.18632/aging.101167
work_keys_str_mv AT mejiaspenayubisay impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT estebanezbrisamar impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT rodriguezmiguelezpaula impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT fernandezgonzalorodrigo impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT almarmar impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT depazjosea impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT gonzalezgallegojavier impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects
AT cuevasmariaj impactofresistancetrainingontheautophagyinflammationapoptosiscrosstalkinelderlysubjects