Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel

Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins using a mix of three different immobilized kinase inhibitors (Purvalanol B, Bisi...

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Autores principales: Petrovic, Voin, Olaisen, Camilla, Sharma, Animesh, Nepal, Anala, Bugge, Steffen, Sundby, Eirik, Hoff, Bård Helge, Slupphaug, Geir, Otterlei, Marit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362150/
https://www.ncbi.nlm.nih.gov/pubmed/28367485
http://dx.doi.org/10.1016/j.dib.2017.03.023
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author Petrovic, Voin
Olaisen, Camilla
Sharma, Animesh
Nepal, Anala
Bugge, Steffen
Sundby, Eirik
Hoff, Bård Helge
Slupphaug, Geir
Otterlei, Marit
author_facet Petrovic, Voin
Olaisen, Camilla
Sharma, Animesh
Nepal, Anala
Bugge, Steffen
Sundby, Eirik
Hoff, Bård Helge
Slupphaug, Geir
Otterlei, Marit
author_sort Petrovic, Voin
collection PubMed
description Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins using a mix of three different immobilized kinase inhibitors (Purvalanol B, Bisindolylmaleimide X, and (R)-3-(4-((1-Phenylethyl)amino)thieno[2,3-d]pyrimidin-6-yl)benzoic acid (SB6-060-05)) on sepharose bead columns. Raw data is deposited in the PRIDE database [1], project number PXD005286. Data presented () shows changes relative to untreated control for each biological replicate for the three cell lines.
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spelling pubmed-53621502017-03-31 Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel Petrovic, Voin Olaisen, Camilla Sharma, Animesh Nepal, Anala Bugge, Steffen Sundby, Eirik Hoff, Bård Helge Slupphaug, Geir Otterlei, Marit Data Brief Data Article Cell extracts from A549, H460, and U2OS human cancer cell lines treated with cisplatin and docetaxel were analyzed by mass spectrometry (MS) proteomic analysis. The extracts were enriched for cellular signaling proteins using a mix of three different immobilized kinase inhibitors (Purvalanol B, Bisindolylmaleimide X, and (R)-3-(4-((1-Phenylethyl)amino)thieno[2,3-d]pyrimidin-6-yl)benzoic acid (SB6-060-05)) on sepharose bead columns. Raw data is deposited in the PRIDE database [1], project number PXD005286. Data presented () shows changes relative to untreated control for each biological replicate for the three cell lines. Elsevier 2017-03-18 /pmc/articles/PMC5362150/ /pubmed/28367485 http://dx.doi.org/10.1016/j.dib.2017.03.023 Text en © Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Petrovic, Voin
Olaisen, Camilla
Sharma, Animesh
Nepal, Anala
Bugge, Steffen
Sundby, Eirik
Hoff, Bård Helge
Slupphaug, Geir
Otterlei, Marit
Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
title Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
title_full Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
title_fullStr Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
title_full_unstemmed Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
title_short Changes in cellular signaling proteins in extracts from A549, H460, and U2OS cells treated with cisplatin or docetaxel
title_sort changes in cellular signaling proteins in extracts from a549, h460, and u2os cells treated with cisplatin or docetaxel
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362150/
https://www.ncbi.nlm.nih.gov/pubmed/28367485
http://dx.doi.org/10.1016/j.dib.2017.03.023
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