Cargando…
miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole
PURPOSE: The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group. METHODS: In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreou...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362220/ https://www.ncbi.nlm.nih.gov/pubmed/28328945 http://dx.doi.org/10.1371/journal.pone.0174297 |
_version_ | 1782516923861303296 |
---|---|
author | Russo, Andrea Ragusa, Marco Barbagallo, Cristina Longo, Antonio Avitabile, Teresio Uva, Maurizio G. Bonfiglio, Vincenza Toro, Mario D. Caltabiano, Rosario Mariotti, Cesare Boscia, Francesco Romano, Mario Di Pietro, Cinzia Barbagallo, Davide Purrello, Michele Reibaldi, Michele |
author_facet | Russo, Andrea Ragusa, Marco Barbagallo, Cristina Longo, Antonio Avitabile, Teresio Uva, Maurizio G. Bonfiglio, Vincenza Toro, Mario D. Caltabiano, Rosario Mariotti, Cesare Boscia, Francesco Romano, Mario Di Pietro, Cinzia Barbagallo, Davide Purrello, Michele Reibaldi, Michele |
author_sort | Russo, Andrea |
collection | PubMed |
description | PURPOSE: The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group. METHODS: In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan(®) Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan(®) assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors. RESULTS: Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan(®) assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p:<0.00004), mir-24 (FC -7.52, p:<0.004) and miR-142-3p (FC -5.32, p:<0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM. CONCLUSION: The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM. |
format | Online Article Text |
id | pubmed-5362220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53622202017-04-06 miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole Russo, Andrea Ragusa, Marco Barbagallo, Cristina Longo, Antonio Avitabile, Teresio Uva, Maurizio G. Bonfiglio, Vincenza Toro, Mario D. Caltabiano, Rosario Mariotti, Cesare Boscia, Francesco Romano, Mario Di Pietro, Cinzia Barbagallo, Davide Purrello, Michele Reibaldi, Michele PLoS One Research Article PURPOSE: The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group. METHODS: In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan(®) Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan(®) assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors. RESULTS: Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan(®) assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p:<0.00004), mir-24 (FC -7.52, p:<0.004) and miR-142-3p (FC -5.32, p:<0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM. CONCLUSION: The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM. Public Library of Science 2017-03-22 /pmc/articles/PMC5362220/ /pubmed/28328945 http://dx.doi.org/10.1371/journal.pone.0174297 Text en © 2017 Russo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Russo, Andrea Ragusa, Marco Barbagallo, Cristina Longo, Antonio Avitabile, Teresio Uva, Maurizio G. Bonfiglio, Vincenza Toro, Mario D. Caltabiano, Rosario Mariotti, Cesare Boscia, Francesco Romano, Mario Di Pietro, Cinzia Barbagallo, Davide Purrello, Michele Reibaldi, Michele miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
title | miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
title_full | miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
title_fullStr | miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
title_full_unstemmed | miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
title_short | miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
title_sort | mirnas in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362220/ https://www.ncbi.nlm.nih.gov/pubmed/28328945 http://dx.doi.org/10.1371/journal.pone.0174297 |
work_keys_str_mv | AT russoandrea mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT ragusamarco mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT barbagallocristina mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT longoantonio mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT avitabileteresio mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT uvamauriziog mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT bonfigliovincenza mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT toromariod mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT caltabianorosario mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT mariotticesare mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT bosciafrancesco mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT romanomario mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT dipietrocinzia mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT barbagallodavide mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT purrellomichele mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole AT reibaldimichele mirnasinthevitreoushumorofpatientsaffectedbyidiopathicepiretinalmembraneandmacularhole |