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MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ
CCAAT/enhancer-binding protein beta (C/EBPβ) is a transcription factor controlling a broad range of genes essential for homeostasis, including genes related to immune functions, inflammation, metabolism and growth. Monocyte chemoattractant protein-1-induced protein 1 (MCPIP1) also called as Regnase-...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362230/ https://www.ncbi.nlm.nih.gov/pubmed/28328949 http://dx.doi.org/10.1371/journal.pone.0174381 |
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author | Lipert, Barbara Wilamowski, Mateusz Gorecki, Andrzej Jura, Jolanta |
author_facet | Lipert, Barbara Wilamowski, Mateusz Gorecki, Andrzej Jura, Jolanta |
author_sort | Lipert, Barbara |
collection | PubMed |
description | CCAAT/enhancer-binding protein beta (C/EBPβ) is a transcription factor controlling a broad range of genes essential for homeostasis, including genes related to immune functions, inflammation, metabolism and growth. Monocyte chemoattractant protein-1-induced protein 1 (MCPIP1) also called as Regnase-1 is an RNase and has been shown to decrease the stability of short-lived transcripts coding for inflammation-related proteins, including IL-1β, IL-6, IL-2, IL-8, IL-12b, IER-3, c-Rel. We found previously that the half-life of the C/EBPβ transcript is regulated by MCPIP. To understand the mechanism driving down-regulation of C/EBPβ by MCPIP1, we applied an in vitro cleavage assay, followed by a luciferase-reporter assay and RNA immunoprecipitation (RIP). We demonstrated that MCPIP1 recognizes regions of the 3’UTR of C/EBPβ mRNA and promotes its decay by introducing direct endonucleolytic cleavage. |
format | Online Article Text |
id | pubmed-5362230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53622302017-04-06 MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ Lipert, Barbara Wilamowski, Mateusz Gorecki, Andrzej Jura, Jolanta PLoS One Research Article CCAAT/enhancer-binding protein beta (C/EBPβ) is a transcription factor controlling a broad range of genes essential for homeostasis, including genes related to immune functions, inflammation, metabolism and growth. Monocyte chemoattractant protein-1-induced protein 1 (MCPIP1) also called as Regnase-1 is an RNase and has been shown to decrease the stability of short-lived transcripts coding for inflammation-related proteins, including IL-1β, IL-6, IL-2, IL-8, IL-12b, IER-3, c-Rel. We found previously that the half-life of the C/EBPβ transcript is regulated by MCPIP. To understand the mechanism driving down-regulation of C/EBPβ by MCPIP1, we applied an in vitro cleavage assay, followed by a luciferase-reporter assay and RNA immunoprecipitation (RIP). We demonstrated that MCPIP1 recognizes regions of the 3’UTR of C/EBPβ mRNA and promotes its decay by introducing direct endonucleolytic cleavage. Public Library of Science 2017-03-22 /pmc/articles/PMC5362230/ /pubmed/28328949 http://dx.doi.org/10.1371/journal.pone.0174381 Text en © 2017 Lipert et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lipert, Barbara Wilamowski, Mateusz Gorecki, Andrzej Jura, Jolanta MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ |
title | MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ |
title_full | MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ |
title_fullStr | MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ |
title_full_unstemmed | MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ |
title_short | MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ |
title_sort | mcpip1, alias regnase-1 binds and cleaves mrna of c/ebpβ |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362230/ https://www.ncbi.nlm.nih.gov/pubmed/28328949 http://dx.doi.org/10.1371/journal.pone.0174381 |
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