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Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition

The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid drople...

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Autores principales: Fan, Wei, Lam, Sin Man, Xin, Jingxue, Yang, Xiao, Liu, Zhonghua, Liu, Yuan, Wang, Yong, Shui, Guanghou, Huang, Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362240/
https://www.ncbi.nlm.nih.gov/pubmed/28273089
http://dx.doi.org/10.1371/journal.pgen.1006664
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author Fan, Wei
Lam, Sin Man
Xin, Jingxue
Yang, Xiao
Liu, Zhonghua
Liu, Yuan
Wang, Yong
Shui, Guanghou
Huang, Xun
author_facet Fan, Wei
Lam, Sin Man
Xin, Jingxue
Yang, Xiao
Liu, Zhonghua
Liu, Yuan
Wang, Yong
Shui, Guanghou
Huang, Xun
author_sort Fan, Wei
collection PubMed
description The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.
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spelling pubmed-53622402017-04-06 Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition Fan, Wei Lam, Sin Man Xin, Jingxue Yang, Xiao Liu, Zhonghua Liu, Yuan Wang, Yong Shui, Guanghou Huang, Xun PLoS Genet Research Article The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis. Public Library of Science 2017-03-08 /pmc/articles/PMC5362240/ /pubmed/28273089 http://dx.doi.org/10.1371/journal.pgen.1006664 Text en © 2017 Fan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fan, Wei
Lam, Sin Man
Xin, Jingxue
Yang, Xiao
Liu, Zhonghua
Liu, Yuan
Wang, Yong
Shui, Guanghou
Huang, Xun
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
title Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
title_full Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
title_fullStr Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
title_full_unstemmed Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
title_short Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
title_sort drosophila trf2 and taf9 regulate lipid droplet size and phospholipid fatty acid composition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362240/
https://www.ncbi.nlm.nih.gov/pubmed/28273089
http://dx.doi.org/10.1371/journal.pgen.1006664
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