Cargando…

Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis

The incidence of rheumatoid arthritis (RA) has been reported to be correlated with a disorder of immunregulation. Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) play an important role in regulating the local immune microenvironment. However, the potential mechanism of RA-FLS in regulati...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Ru, Zhang, Yingzi, Wang, Lin, Hu, Ji, Wen, Jian, Xue, Leixi, Tang, Mei, Liu, Zhichun, Fu, Jinxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362496/
https://www.ncbi.nlm.nih.gov/pubmed/28053286
http://dx.doi.org/10.18632/oncotarget.14331
_version_ 1782516962991013888
author Yang, Ru
Zhang, Yingzi
Wang, Lin
Hu, Ji
Wen, Jian
Xue, Leixi
Tang, Mei
Liu, Zhichun
Fu, Jinxiang
author_facet Yang, Ru
Zhang, Yingzi
Wang, Lin
Hu, Ji
Wen, Jian
Xue, Leixi
Tang, Mei
Liu, Zhichun
Fu, Jinxiang
author_sort Yang, Ru
collection PubMed
description The incidence of rheumatoid arthritis (RA) has been reported to be correlated with a disorder of immunregulation. Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) play an important role in regulating the local immune microenvironment. However, the potential mechanism of RA-FLS in regulating the immnue response is not clearly understood. In this study, we demonstrated that the expression of HIF-1α was significantly up-regulated in rheumatoid arthritis tissue which indicated that the hypoxia condition in the microenvironment. We also observed that RA-FLSs demonstrated the potential to up-regulate immune activation. Meanwhile, the level of autophagy increased in RA-FLSs compared with control group. Besides that, the expression of IL-6 was up-regulated not only in RA-FLSs but also in the fibroblasts that treated with hypoxia condition. Accordingly, we found that autophagy inhibitiors could effectively inhibit the immune activation function of RA-FLSs medicated by IL-6. Taken together, the results we demonstrated above indicated that the hypoxia microenvironment could effectively induce the incidence of autophagy and then lead to the immune activation function of RA-FLSs medicated by IL-6.
format Online
Article
Text
id pubmed-5362496
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-53624962017-04-24 Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis Yang, Ru Zhang, Yingzi Wang, Lin Hu, Ji Wen, Jian Xue, Leixi Tang, Mei Liu, Zhichun Fu, Jinxiang Oncotarget Research Paper The incidence of rheumatoid arthritis (RA) has been reported to be correlated with a disorder of immunregulation. Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) play an important role in regulating the local immune microenvironment. However, the potential mechanism of RA-FLS in regulating the immnue response is not clearly understood. In this study, we demonstrated that the expression of HIF-1α was significantly up-regulated in rheumatoid arthritis tissue which indicated that the hypoxia condition in the microenvironment. We also observed that RA-FLSs demonstrated the potential to up-regulate immune activation. Meanwhile, the level of autophagy increased in RA-FLSs compared with control group. Besides that, the expression of IL-6 was up-regulated not only in RA-FLSs but also in the fibroblasts that treated with hypoxia condition. Accordingly, we found that autophagy inhibitiors could effectively inhibit the immune activation function of RA-FLSs medicated by IL-6. Taken together, the results we demonstrated above indicated that the hypoxia microenvironment could effectively induce the incidence of autophagy and then lead to the immune activation function of RA-FLSs medicated by IL-6. Impact Journals LLC 2016-12-28 /pmc/articles/PMC5362496/ /pubmed/28053286 http://dx.doi.org/10.18632/oncotarget.14331 Text en Copyright: © 2017 Yang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yang, Ru
Zhang, Yingzi
Wang, Lin
Hu, Ji
Wen, Jian
Xue, Leixi
Tang, Mei
Liu, Zhichun
Fu, Jinxiang
Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
title Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
title_full Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
title_fullStr Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
title_full_unstemmed Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
title_short Increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
title_sort increased autophagy in fibroblast-like synoviocytes leads to immune enhancement potential in rheumatoid arthritis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362496/
https://www.ncbi.nlm.nih.gov/pubmed/28053286
http://dx.doi.org/10.18632/oncotarget.14331
work_keys_str_mv AT yangru increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT zhangyingzi increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT wanglin increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT huji increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT wenjian increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT xueleixi increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT tangmei increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT liuzhichun increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis
AT fujinxiang increasedautophagyinfibroblastlikesynoviocytesleadstoimmuneenhancementpotentialinrheumatoidarthritis