Cargando…
Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells
Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44(bright)/CD24...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362508/ https://www.ncbi.nlm.nih.gov/pubmed/28121626 http://dx.doi.org/10.18632/oncotarget.14777 |
_version_ | 1782516965993086976 |
---|---|
author | Hsieh, Chin-Hsuan Hsiung, Shih-Chieh Yeh, Chi-Tai Yen, Chih-Feng Chou, Yah-Huei Wu Lei, Wei-Yi Pang, See-Tong Chuang, Cheng-Keng Liao, Shuen-Kuei |
author_facet | Hsieh, Chin-Hsuan Hsiung, Shih-Chieh Yeh, Chi-Tai Yen, Chih-Feng Chou, Yah-Huei Wu Lei, Wei-Yi Pang, See-Tong Chuang, Cheng-Keng Liao, Shuen-Kuei |
author_sort | Hsieh, Chin-Hsuan |
collection | PubMed |
description | Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44(bright)/CD24(dim) and CD44(bright)/CD24(bright) phenotypes correspond to epithelioid and fibroblastoid subsets, respectively. Both sorted subsets displayed different levels of tumorigenicity in xenotransplantation, indicating that each harbored its own cancer stem cells (CSCs). The CD44(bright)/CD24(bright) subset, associated with higher expression of MMP-7, -8 and TIMP-1 transcripts, showed greater migratory/invasive potential than the CD44(bright)/CD24(dim) subset, which was associated with higher expression of MMP-2, -9 and TIMP-2 transcripts. Both subsets differentially expressed stemness gene products c-Myc, Oct4A, Notch1, Notch2 and Notch3, and the RCC stem cell marker, CD105 in 4-5% of RCC52 cells. These results suggest the presence of CSCs in both sRCC subsets for the first time and should therefore be considered potential therapeutic targets for this aggressive malignancy. |
format | Online Article Text |
id | pubmed-5362508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53625082017-04-24 Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells Hsieh, Chin-Hsuan Hsiung, Shih-Chieh Yeh, Chi-Tai Yen, Chih-Feng Chou, Yah-Huei Wu Lei, Wei-Yi Pang, See-Tong Chuang, Cheng-Keng Liao, Shuen-Kuei Oncotarget Research Paper Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44(bright)/CD24(dim) and CD44(bright)/CD24(bright) phenotypes correspond to epithelioid and fibroblastoid subsets, respectively. Both sorted subsets displayed different levels of tumorigenicity in xenotransplantation, indicating that each harbored its own cancer stem cells (CSCs). The CD44(bright)/CD24(bright) subset, associated with higher expression of MMP-7, -8 and TIMP-1 transcripts, showed greater migratory/invasive potential than the CD44(bright)/CD24(dim) subset, which was associated with higher expression of MMP-2, -9 and TIMP-2 transcripts. Both subsets differentially expressed stemness gene products c-Myc, Oct4A, Notch1, Notch2 and Notch3, and the RCC stem cell marker, CD105 in 4-5% of RCC52 cells. These results suggest the presence of CSCs in both sRCC subsets for the first time and should therefore be considered potential therapeutic targets for this aggressive malignancy. Impact Journals LLC 2017-01-21 /pmc/articles/PMC5362508/ /pubmed/28121626 http://dx.doi.org/10.18632/oncotarget.14777 Text en Copyright: © 2017 Hsieh et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Hsieh, Chin-Hsuan Hsiung, Shih-Chieh Yeh, Chi-Tai Yen, Chih-Feng Chou, Yah-Huei Wu Lei, Wei-Yi Pang, See-Tong Chuang, Cheng-Keng Liao, Shuen-Kuei Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
title | Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
title_full | Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
title_fullStr | Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
title_full_unstemmed | Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
title_short | Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
title_sort | differential expression of cd44 and cd24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362508/ https://www.ncbi.nlm.nih.gov/pubmed/28121626 http://dx.doi.org/10.18632/oncotarget.14777 |
work_keys_str_mv | AT hsiehchinhsuan differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT hsiungshihchieh differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT yehchitai differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT yenchihfeng differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT chouyahhueiwu differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT leiweiyi differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT pangseetong differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT chuangchengkeng differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells AT liaoshuenkuei differentialexpressionofcd44andcd24markersdiscriminatestheepitheliodfromthefibroblastoidsubsetinasarcomatoidrenalcarcinomacelllineevidencesuggestingtheexistenceofcancerstemcellsinbothsubsetsasstudiedwithsortedcells |