Next-Generation Sequencing Combined with Specific PCR Assays To Determine the Bacterial 16S rRNA Gene Profiles of Middle Ear Fluid Collected from Children with Acute Otitis Media

The aim of the study was to analyze the bacteriome of acute otitis media with a novel modification of next-generation sequencing techniques. Outpatient children with acute otitis media were enrolled in the study, and middle ear fluids were collected during 90 episodes from 79 subjects aged 5 to 42 m...

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Detalles Bibliográficos
Autores principales: Sillanpää, Saara, Kramna, Lenka, Oikarinen, Sami, Sipilä, Markku, Rautiainen, Markus, Aittoniemi, Janne, Laranne, Jussi, Hyöty, Heikki, Cinek, Ondrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362748/
https://www.ncbi.nlm.nih.gov/pubmed/28357413
http://dx.doi.org/10.1128/mSphere.00006-17

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