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Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs

Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopep...

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Autores principales: Chen, Yen-Ming, Chen, Li-Hua, Li, Meng-Pei, Li, Hsing-Fen, Higuchi, Akon, Kumar, S. Suresh, Ling, Qing-Dong, Alarfaj, Abdullah A., Munusamy, Murugan A., Chang, Yung, Benelli, Giovanni, Murugan, Kadarkarai, Umezawa, Akihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362828/
https://www.ncbi.nlm.nih.gov/pubmed/28332572
http://dx.doi.org/10.1038/srep45146
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author Chen, Yen-Ming
Chen, Li-Hua
Li, Meng-Pei
Li, Hsing-Fen
Higuchi, Akon
Kumar, S. Suresh
Ling, Qing-Dong
Alarfaj, Abdullah A.
Munusamy, Murugan A.
Chang, Yung
Benelli, Giovanni
Murugan, Kadarkarai
Umezawa, Akihiro
author_facet Chen, Yen-Ming
Chen, Li-Hua
Li, Meng-Pei
Li, Hsing-Fen
Higuchi, Akon
Kumar, S. Suresh
Ling, Qing-Dong
Alarfaj, Abdullah A.
Munusamy, Murugan A.
Chang, Yung
Benelli, Giovanni
Murugan, Kadarkarai
Umezawa, Akihiro
author_sort Chen, Yen-Ming
collection PubMed
description Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain. Oligopeptide sequences were selected from integrin- and glycosaminoglycan-binding domains of the extracellular matrix. The hydrogels grafted with vitronectin-derived oligopeptides having a joint segment or a dual chain, which has a storage modulus of 25 kPa, supported the long-term culture of human ES and iPS cells for over 10 passages. The dual chain and/or joint segment with cell adhesion molecules on the hydrogels facilitated the proliferation and pluripotency of human ES and iPS cells.
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spelling pubmed-53628282017-03-24 Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs Chen, Yen-Ming Chen, Li-Hua Li, Meng-Pei Li, Hsing-Fen Higuchi, Akon Kumar, S. Suresh Ling, Qing-Dong Alarfaj, Abdullah A. Munusamy, Murugan A. Chang, Yung Benelli, Giovanni Murugan, Kadarkarai Umezawa, Akihiro Sci Rep Article Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain. Oligopeptide sequences were selected from integrin- and glycosaminoglycan-binding domains of the extracellular matrix. The hydrogels grafted with vitronectin-derived oligopeptides having a joint segment or a dual chain, which has a storage modulus of 25 kPa, supported the long-term culture of human ES and iPS cells for over 10 passages. The dual chain and/or joint segment with cell adhesion molecules on the hydrogels facilitated the proliferation and pluripotency of human ES and iPS cells. Nature Publishing Group 2017-03-23 /pmc/articles/PMC5362828/ /pubmed/28332572 http://dx.doi.org/10.1038/srep45146 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Yen-Ming
Chen, Li-Hua
Li, Meng-Pei
Li, Hsing-Fen
Higuchi, Akon
Kumar, S. Suresh
Ling, Qing-Dong
Alarfaj, Abdullah A.
Munusamy, Murugan A.
Chang, Yung
Benelli, Giovanni
Murugan, Kadarkarai
Umezawa, Akihiro
Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
title Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
title_full Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
title_fullStr Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
title_full_unstemmed Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
title_short Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
title_sort xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362828/
https://www.ncbi.nlm.nih.gov/pubmed/28332572
http://dx.doi.org/10.1038/srep45146
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