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Global preamplification simplifies targeted mRNA quantification
The need to perform gene expression profiling using next generation sequencing and quantitative real-time PCR (qPCR) on small sample sizes and single cells is rapidly expanding. However, to analyse few molecules, preamplification is required. Here, we studied global and target-specific preamplificat...
Autores principales: | Kroneis, Thomas, Jonasson, Emma, Andersson, Daniel, Dolatabadi, Soheila, Ståhlberg, Anders |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362892/ https://www.ncbi.nlm.nih.gov/pubmed/28332609 http://dx.doi.org/10.1038/srep45219 |
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