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Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig

BACKGROUND: Cigarette smoke (CS) is associated with lower numbers of circulating stem cells and might severely affect their mobilization, trafficking and homing. Our study was designed to demonstrate in an animal model of CS exposure whether CS affects the homing and functional capabilities of bone...

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Autores principales: Tura-Ceide, Olga, Lobo, Borja, Paul, Tanja, Puig-Pey, Raquel, Coll-Bonfill, Núria, García-Lucio, Jéssica, Smolders, Valérie, Blanco, Isabel, Barberà, Joan A., Peinado, Víctor I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5363047/
https://www.ncbi.nlm.nih.gov/pubmed/28330488
http://dx.doi.org/10.1186/s12931-017-0530-0
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author Tura-Ceide, Olga
Lobo, Borja
Paul, Tanja
Puig-Pey, Raquel
Coll-Bonfill, Núria
García-Lucio, Jéssica
Smolders, Valérie
Blanco, Isabel
Barberà, Joan A.
Peinado, Víctor I.
author_facet Tura-Ceide, Olga
Lobo, Borja
Paul, Tanja
Puig-Pey, Raquel
Coll-Bonfill, Núria
García-Lucio, Jéssica
Smolders, Valérie
Blanco, Isabel
Barberà, Joan A.
Peinado, Víctor I.
author_sort Tura-Ceide, Olga
collection PubMed
description BACKGROUND: Cigarette smoke (CS) is associated with lower numbers of circulating stem cells and might severely affect their mobilization, trafficking and homing. Our study was designed to demonstrate in an animal model of CS exposure whether CS affects the homing and functional capabilities of bone marrow-derived mesenchymal stem cells (BM-MSCs). METHODS: Guinea pigs (GP), exposed or sham-exposed to CS, were administered via tracheal instillation or by vascular administration with 2.5 × 10(6) BM-MSCs obtained from CS-exposed or sham-exposed animal donors. Twenty-four hours after cell administration, animals were sacrificed and cells were visualised into lung structures by optical microscopy. BM-MSCs from 8 healthy GP and from 8 GP exposed to CS for 1 month were isolated from the femur, cultured in vitro and assessed for their proliferation, migration, senescence, differentiation potential and chemokine gene expression profile. RESULTS: CS-exposed animals showed greater BM-MSCs lung infiltration than sham-exposed animals regardless of route of administration. The majority of BM-MSCs localized in the alveolar septa. BM-MSCs obtained from CS-exposed animals showed lower ability to engraft and lower proliferation and migration. In vitro, BM-MSCs exposed to CS extract showed a significant reduction of proliferative, cellular differentiation and migratory potential and an increase in cellular senescence in a dose dependent manner. CONCLUSION: Short-term CS exposure induces BM-MSCs dysfunction. Such dysfunction was observed in vivo, affecting the cell homing and proliferation capabilities of BM-MSCs in lungs exposed to CS and in vitro altering the rate of proliferation, senescence, differentiation and migration capacity. Additionally, CS induced a reduction in CXCL9 gene expression in the BM from CS-exposed animals underpinning a potential mechanistic action of bone marrow dysfunction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12931-017-0530-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-53630472017-03-24 Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig Tura-Ceide, Olga Lobo, Borja Paul, Tanja Puig-Pey, Raquel Coll-Bonfill, Núria García-Lucio, Jéssica Smolders, Valérie Blanco, Isabel Barberà, Joan A. Peinado, Víctor I. Respir Res Research BACKGROUND: Cigarette smoke (CS) is associated with lower numbers of circulating stem cells and might severely affect their mobilization, trafficking and homing. Our study was designed to demonstrate in an animal model of CS exposure whether CS affects the homing and functional capabilities of bone marrow-derived mesenchymal stem cells (BM-MSCs). METHODS: Guinea pigs (GP), exposed or sham-exposed to CS, were administered via tracheal instillation or by vascular administration with 2.5 × 10(6) BM-MSCs obtained from CS-exposed or sham-exposed animal donors. Twenty-four hours after cell administration, animals were sacrificed and cells were visualised into lung structures by optical microscopy. BM-MSCs from 8 healthy GP and from 8 GP exposed to CS for 1 month were isolated from the femur, cultured in vitro and assessed for their proliferation, migration, senescence, differentiation potential and chemokine gene expression profile. RESULTS: CS-exposed animals showed greater BM-MSCs lung infiltration than sham-exposed animals regardless of route of administration. The majority of BM-MSCs localized in the alveolar septa. BM-MSCs obtained from CS-exposed animals showed lower ability to engraft and lower proliferation and migration. In vitro, BM-MSCs exposed to CS extract showed a significant reduction of proliferative, cellular differentiation and migratory potential and an increase in cellular senescence in a dose dependent manner. CONCLUSION: Short-term CS exposure induces BM-MSCs dysfunction. Such dysfunction was observed in vivo, affecting the cell homing and proliferation capabilities of BM-MSCs in lungs exposed to CS and in vitro altering the rate of proliferation, senescence, differentiation and migration capacity. Additionally, CS induced a reduction in CXCL9 gene expression in the BM from CS-exposed animals underpinning a potential mechanistic action of bone marrow dysfunction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12931-017-0530-0) contains supplementary material, which is available to authorized users. BioMed Central 2017-03-23 2017 /pmc/articles/PMC5363047/ /pubmed/28330488 http://dx.doi.org/10.1186/s12931-017-0530-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Tura-Ceide, Olga
Lobo, Borja
Paul, Tanja
Puig-Pey, Raquel
Coll-Bonfill, Núria
García-Lucio, Jéssica
Smolders, Valérie
Blanco, Isabel
Barberà, Joan A.
Peinado, Víctor I.
Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
title Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
title_full Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
title_fullStr Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
title_full_unstemmed Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
title_short Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
title_sort cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5363047/
https://www.ncbi.nlm.nih.gov/pubmed/28330488
http://dx.doi.org/10.1186/s12931-017-0530-0
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