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The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides
Cell-penetrating peptides (CPPs) are delivery vectors widely used to aid the transport of biologically active cargoes to intracellular targets. These cargoes include small interfering RNAs (siRNA) that are not naturally internalized by cells. Elucidating the complexities behind the formation of CPP...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5363680/ https://www.ncbi.nlm.nih.gov/pubmed/28624185 http://dx.doi.org/10.1016/j.omtn.2017.02.003 |
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author | Pärnaste, Ly Arukuusk, Piret Langel, Kent Tenson, Tanel Langel, Ülo |
author_facet | Pärnaste, Ly Arukuusk, Piret Langel, Kent Tenson, Tanel Langel, Ülo |
author_sort | Pärnaste, Ly |
collection | PubMed |
description | Cell-penetrating peptides (CPPs) are delivery vectors widely used to aid the transport of biologically active cargoes to intracellular targets. These cargoes include small interfering RNAs (siRNA) that are not naturally internalized by cells. Elucidating the complexities behind the formation of CPP and cargo complexes is crucial for understanding the processes related to their delivery. In this study, we used modified analogs of the CPP transportan10 and investigated the binding properties of these CPPs to siRNA, the formation parameters of the CPP/siRNA complexes, and their stabiliy to enzymatic degradation. We conclude that the pH dependent change of the net charge of the CPP may very well be the key factor leading to the high delivery efficiency and the optimal binding strength between CPPs to siRNAs, while the hydrophobicity, secondary structure of the CPP, and the positions of the positive charges are responsible for the stability of the CPP/siRNA particles. Also, CPPs with distinct hydrophobic and hydrophilic regions may assemble into nanoparticles that could be described as core-shell formulations. |
format | Online Article Text |
id | pubmed-5363680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-53636802017-03-24 The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides Pärnaste, Ly Arukuusk, Piret Langel, Kent Tenson, Tanel Langel, Ülo Mol Ther Nucleic Acids Original Article Cell-penetrating peptides (CPPs) are delivery vectors widely used to aid the transport of biologically active cargoes to intracellular targets. These cargoes include small interfering RNAs (siRNA) that are not naturally internalized by cells. Elucidating the complexities behind the formation of CPP and cargo complexes is crucial for understanding the processes related to their delivery. In this study, we used modified analogs of the CPP transportan10 and investigated the binding properties of these CPPs to siRNA, the formation parameters of the CPP/siRNA complexes, and their stabiliy to enzymatic degradation. We conclude that the pH dependent change of the net charge of the CPP may very well be the key factor leading to the high delivery efficiency and the optimal binding strength between CPPs to siRNAs, while the hydrophobicity, secondary structure of the CPP, and the positions of the positive charges are responsible for the stability of the CPP/siRNA particles. Also, CPPs with distinct hydrophobic and hydrophilic regions may assemble into nanoparticles that could be described as core-shell formulations. American Society of Gene & Cell Therapy 2017-06-16 2017-02-10 /pmc/articles/PMC5363680/ /pubmed/28624185 http://dx.doi.org/10.1016/j.omtn.2017.02.003 Text en © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Pärnaste, Ly Arukuusk, Piret Langel, Kent Tenson, Tanel Langel, Ülo The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides |
title | The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides |
title_full | The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides |
title_fullStr | The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides |
title_full_unstemmed | The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides |
title_short | The Formation of Nanoparticles between Small Interfering RNA and Amphipathic Cell-Penetrating Peptides |
title_sort | formation of nanoparticles between small interfering rna and amphipathic cell-penetrating peptides |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5363680/ https://www.ncbi.nlm.nih.gov/pubmed/28624185 http://dx.doi.org/10.1016/j.omtn.2017.02.003 |
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