Cargando…

Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles

Biosurfactants, surface-active amphiphilic compounds, despite having a wide range of applications, have a high cost of production, which severely restricts their use. For cheaper production of biosurfactant, we investigated the potential of the indigenously isolated biosurfactant producing organism,...

Descripción completa

Detalles Bibliográficos
Autores principales: Rane, Ashwini N., Baikar, Vishakha V., Ravi Kumar, V., Deopurkar, Rajendra L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364166/
https://www.ncbi.nlm.nih.gov/pubmed/28392783
http://dx.doi.org/10.3389/fmicb.2017.00492
_version_ 1782517269285306368
author Rane, Ashwini N.
Baikar, Vishakha V.
Ravi Kumar, V.
Deopurkar, Rajendra L.
author_facet Rane, Ashwini N.
Baikar, Vishakha V.
Ravi Kumar, V.
Deopurkar, Rajendra L.
author_sort Rane, Ashwini N.
collection PubMed
description Biosurfactants, surface-active amphiphilic compounds, despite having a wide range of applications, have a high cost of production, which severely restricts their use. For cheaper production of biosurfactant, we investigated the potential of the indigenously isolated biosurfactant producing organism, Bacillus subtilis ANR 88, to grow on different cheap carbon sources (molasses, whey, and extracts of potato peels, orange peels, banana peels, and bagasse). We found that, B. subtilis ANR 88 used significant amounts of total sugar to produce cell biomass and biosurfactant. The biosurfactant production in minimal medium containing glucose as sole source of carbon was 0.207 g/l and the same with molasses as carbon source was 0.241 g/l. With whey as carbon source, isolate failed to produce biosurfactant. Amongst the extracts of the agro-wastes, the extracts of bagasse and orange peels gave 0.127 and 0.089 g/l of biosurfactant respectively. One-variable-at-a-time (OVAT) studies carried out to optimize the production of biosurfactant by B. subtilis ANR 88 resulted into maximum biosurfactant yield of 0.513 g/l in medium: molasses 4%, ammonium ferric citrate 0.25%, pH 7. Plackett–Burman design based statistical method for optimization increased the production of biosurfactant to 0.746 g/l, which is 3.6-fold of that produced on glucose. The biosurfactant produced by B. subtilis ANR 88 was analyzed by Fourier Transform Infrared Spectroscopy (FT-IR); it showed that the biosurfactant contained alkyl as well as peptide groups. The biosurfactant of B. subtilis ANR 88 was found effective in the synthesis of silver as well as gold nanoparticles in the total absence of conventional chemical reducing agents. Interestingly, nanoparticles produced were almost uniform in their size and shapes i.e., spherical silver (4–18 nm) and hexagonal gold nanoparticles (40–60 nm), as evident in TEM images.
format Online
Article
Text
id pubmed-5364166
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-53641662017-04-07 Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles Rane, Ashwini N. Baikar, Vishakha V. Ravi Kumar, V. Deopurkar, Rajendra L. Front Microbiol Microbiology Biosurfactants, surface-active amphiphilic compounds, despite having a wide range of applications, have a high cost of production, which severely restricts their use. For cheaper production of biosurfactant, we investigated the potential of the indigenously isolated biosurfactant producing organism, Bacillus subtilis ANR 88, to grow on different cheap carbon sources (molasses, whey, and extracts of potato peels, orange peels, banana peels, and bagasse). We found that, B. subtilis ANR 88 used significant amounts of total sugar to produce cell biomass and biosurfactant. The biosurfactant production in minimal medium containing glucose as sole source of carbon was 0.207 g/l and the same with molasses as carbon source was 0.241 g/l. With whey as carbon source, isolate failed to produce biosurfactant. Amongst the extracts of the agro-wastes, the extracts of bagasse and orange peels gave 0.127 and 0.089 g/l of biosurfactant respectively. One-variable-at-a-time (OVAT) studies carried out to optimize the production of biosurfactant by B. subtilis ANR 88 resulted into maximum biosurfactant yield of 0.513 g/l in medium: molasses 4%, ammonium ferric citrate 0.25%, pH 7. Plackett–Burman design based statistical method for optimization increased the production of biosurfactant to 0.746 g/l, which is 3.6-fold of that produced on glucose. The biosurfactant produced by B. subtilis ANR 88 was analyzed by Fourier Transform Infrared Spectroscopy (FT-IR); it showed that the biosurfactant contained alkyl as well as peptide groups. The biosurfactant of B. subtilis ANR 88 was found effective in the synthesis of silver as well as gold nanoparticles in the total absence of conventional chemical reducing agents. Interestingly, nanoparticles produced were almost uniform in their size and shapes i.e., spherical silver (4–18 nm) and hexagonal gold nanoparticles (40–60 nm), as evident in TEM images. Frontiers Media S.A. 2017-03-24 /pmc/articles/PMC5364166/ /pubmed/28392783 http://dx.doi.org/10.3389/fmicb.2017.00492 Text en Copyright © 2017 Rane, Baikar, Ravi Kumar and Deopurkar. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Rane, Ashwini N.
Baikar, Vishakha V.
Ravi Kumar, V.
Deopurkar, Rajendra L.
Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles
title Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles
title_full Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles
title_fullStr Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles
title_full_unstemmed Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles
title_short Agro-Industrial Wastes for Production of Biosurfactant by Bacillus subtilis ANR 88 and Its Application in Synthesis of Silver and Gold Nanoparticles
title_sort agro-industrial wastes for production of biosurfactant by bacillus subtilis anr 88 and its application in synthesis of silver and gold nanoparticles
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364166/
https://www.ncbi.nlm.nih.gov/pubmed/28392783
http://dx.doi.org/10.3389/fmicb.2017.00492
work_keys_str_mv AT raneashwinin agroindustrialwastesforproductionofbiosurfactantbybacillussubtilisanr88anditsapplicationinsynthesisofsilverandgoldnanoparticles
AT baikarvishakhav agroindustrialwastesforproductionofbiosurfactantbybacillussubtilisanr88anditsapplicationinsynthesisofsilverandgoldnanoparticles
AT ravikumarv agroindustrialwastesforproductionofbiosurfactantbybacillussubtilisanr88anditsapplicationinsynthesisofsilverandgoldnanoparticles
AT deopurkarrajendral agroindustrialwastesforproductionofbiosurfactantbybacillussubtilisanr88anditsapplicationinsynthesisofsilverandgoldnanoparticles