A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria

The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available...

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Detalles Bibliográficos
Autores principales: Mugweru, Julius, Makafe, Gaelle, Cao, Yuanyuan, Zhang, Yang, Wang, Bangxing, Huang, Shaobo, Njire, Moses, Chhotaray, Chiranjibi, Tan, Yaoju, Li, Xinjie, Liu, Jianxiong, Tan, Shouyong, Deng, Jiaoyu, Zhang, Tianyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://www.ncbi.nlm.nih.gov/pubmed/28392781
http://dx.doi.org/10.3389/fmicb.2017.00468
Descripción
Sumario:The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available markers, we have created a cassette containing thiostrepton resistance gene (tsr) for selection in M. tuberculosis and M. bovis BCG, and gentamicin resistance gene (aacC1) for Escherichia coli and M. smegmatis mc(2)155, flanked with dif sequences recognized by the Xer system of mycobacteria. This cassette adds to the limited available selection markers for mycobacteria.

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