A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria

The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available...

Descripción completa

Detalles Bibliográficos
Autores principales: Mugweru, Julius, Makafe, Gaelle, Cao, Yuanyuan, Zhang, Yang, Wang, Bangxing, Huang, Shaobo, Njire, Moses, Chhotaray, Chiranjibi, Tan, Yaoju, Li, Xinjie, Liu, Jianxiong, Tan, Shouyong, Deng, Jiaoyu, Zhang, Tianyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://www.ncbi.nlm.nih.gov/pubmed/28392781
http://dx.doi.org/10.3389/fmicb.2017.00468
_version_ 1782517273247875072
author Mugweru, Julius
Makafe, Gaelle
Cao, Yuanyuan
Zhang, Yang
Wang, Bangxing
Huang, Shaobo
Njire, Moses
Chhotaray, Chiranjibi
Tan, Yaoju
Li, Xinjie
Liu, Jianxiong
Tan, Shouyong
Deng, Jiaoyu
Zhang, Tianyu
author_facet Mugweru, Julius
Makafe, Gaelle
Cao, Yuanyuan
Zhang, Yang
Wang, Bangxing
Huang, Shaobo
Njire, Moses
Chhotaray, Chiranjibi
Tan, Yaoju
Li, Xinjie
Liu, Jianxiong
Tan, Shouyong
Deng, Jiaoyu
Zhang, Tianyu
author_sort Mugweru, Julius
collection PubMed
description The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available markers, we have created a cassette containing thiostrepton resistance gene (tsr) for selection in M. tuberculosis and M. bovis BCG, and gentamicin resistance gene (aacC1) for Escherichia coli and M. smegmatis mc(2)155, flanked with dif sequences recognized by the Xer system of mycobacteria. This cassette adds to the limited available selection markers for mycobacteria.
format Online
Article
Text
id pubmed-5364183
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-53641832017-04-07 A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria Mugweru, Julius Makafe, Gaelle Cao, Yuanyuan Zhang, Yang Wang, Bangxing Huang, Shaobo Njire, Moses Chhotaray, Chiranjibi Tan, Yaoju Li, Xinjie Liu, Jianxiong Tan, Shouyong Deng, Jiaoyu Zhang, Tianyu Front Microbiol Microbiology The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available markers, we have created a cassette containing thiostrepton resistance gene (tsr) for selection in M. tuberculosis and M. bovis BCG, and gentamicin resistance gene (aacC1) for Escherichia coli and M. smegmatis mc(2)155, flanked with dif sequences recognized by the Xer system of mycobacteria. This cassette adds to the limited available selection markers for mycobacteria. Frontiers Media S.A. 2017-03-24 /pmc/articles/PMC5364183/ /pubmed/28392781 http://dx.doi.org/10.3389/fmicb.2017.00468 Text en Copyright © 2017 Mugweru, Makafe, Cao, Zhang, Wang, Huang, Njire, Chhotaray, Tan, Li, Liu, Tan, Deng and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mugweru, Julius
Makafe, Gaelle
Cao, Yuanyuan
Zhang, Yang
Wang, Bangxing
Huang, Shaobo
Njire, Moses
Chhotaray, Chiranjibi
Tan, Yaoju
Li, Xinjie
Liu, Jianxiong
Tan, Shouyong
Deng, Jiaoyu
Zhang, Tianyu
A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
title A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
title_full A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
title_fullStr A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
title_full_unstemmed A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
title_short A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
title_sort cassette containing thiostrepton, gentamicin resistance genes, and dif sequences is effective in construction of recombinant mycobacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364183/
https://www.ncbi.nlm.nih.gov/pubmed/28392781
http://dx.doi.org/10.3389/fmicb.2017.00468
work_keys_str_mv AT mugwerujulius acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT makafegaelle acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT caoyuanyuan acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT zhangyang acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT wangbangxing acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT huangshaobo acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT njiremoses acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT chhotaraychiranjibi acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT tanyaoju acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT lixinjie acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT liujianxiong acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT tanshouyong acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT dengjiaoyu acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT zhangtianyu acassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT mugwerujulius cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT makafegaelle cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT caoyuanyuan cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT zhangyang cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT wangbangxing cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT huangshaobo cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT njiremoses cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT chhotaraychiranjibi cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT tanyaoju cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT lixinjie cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT liujianxiong cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT tanshouyong cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT dengjiaoyu cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria
AT zhangtianyu cassettecontainingthiostreptongentamicinresistancegenesanddifsequencesiseffectiveinconstructionofrecombinantmycobacteria

Ejemplares similares