Cargando…

Origins of chemoreceptor curvature sorting in Escherichia coli

Bacterial chemoreceptors organize into large clusters at the cell poles. Despite a wealth of structural and biochemical information on the system's components, it is not clear how chemoreceptor clusters are reliably targeted to the cell pole. Here, we quantify the curvature-dependent localizati...

Descripción completa

Detalles Bibliográficos
Autores principales: Draper, Will, Liphardt, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364426/
https://www.ncbi.nlm.nih.gov/pubmed/28322223
http://dx.doi.org/10.1038/ncomms14838
_version_ 1782517316039213056
author Draper, Will
Liphardt, Jan
author_facet Draper, Will
Liphardt, Jan
author_sort Draper, Will
collection PubMed
description Bacterial chemoreceptors organize into large clusters at the cell poles. Despite a wealth of structural and biochemical information on the system's components, it is not clear how chemoreceptor clusters are reliably targeted to the cell pole. Here, we quantify the curvature-dependent localization of chemoreceptors in live cells by artificially deforming growing cells of Escherichia coli in curved agar microchambers, and find that chemoreceptor cluster localization is highly sensitive to membrane curvature. Through analysis of multiple mutants, we conclude that curvature sensitivity is intrinsic to chemoreceptor trimers-of-dimers, and results from conformational entropy within the trimer-of-dimers geometry. We use the principles of the conformational entropy model to engineer curvature sensitivity into a series of multi-component synthetic protein complexes. When expressed in E. coli, the synthetic complexes form large polar clusters, and a complex with inverted geometry avoids the cell poles. This demonstrates the successful rational design of both polar and anti-polar clustering, and provides a synthetic platform on which to build new systems.
format Online
Article
Text
id pubmed-5364426
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-53644262017-04-11 Origins of chemoreceptor curvature sorting in Escherichia coli Draper, Will Liphardt, Jan Nat Commun Article Bacterial chemoreceptors organize into large clusters at the cell poles. Despite a wealth of structural and biochemical information on the system's components, it is not clear how chemoreceptor clusters are reliably targeted to the cell pole. Here, we quantify the curvature-dependent localization of chemoreceptors in live cells by artificially deforming growing cells of Escherichia coli in curved agar microchambers, and find that chemoreceptor cluster localization is highly sensitive to membrane curvature. Through analysis of multiple mutants, we conclude that curvature sensitivity is intrinsic to chemoreceptor trimers-of-dimers, and results from conformational entropy within the trimer-of-dimers geometry. We use the principles of the conformational entropy model to engineer curvature sensitivity into a series of multi-component synthetic protein complexes. When expressed in E. coli, the synthetic complexes form large polar clusters, and a complex with inverted geometry avoids the cell poles. This demonstrates the successful rational design of both polar and anti-polar clustering, and provides a synthetic platform on which to build new systems. Nature Publishing Group 2017-03-21 /pmc/articles/PMC5364426/ /pubmed/28322223 http://dx.doi.org/10.1038/ncomms14838 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Draper, Will
Liphardt, Jan
Origins of chemoreceptor curvature sorting in Escherichia coli
title Origins of chemoreceptor curvature sorting in Escherichia coli
title_full Origins of chemoreceptor curvature sorting in Escherichia coli
title_fullStr Origins of chemoreceptor curvature sorting in Escherichia coli
title_full_unstemmed Origins of chemoreceptor curvature sorting in Escherichia coli
title_short Origins of chemoreceptor curvature sorting in Escherichia coli
title_sort origins of chemoreceptor curvature sorting in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364426/
https://www.ncbi.nlm.nih.gov/pubmed/28322223
http://dx.doi.org/10.1038/ncomms14838
work_keys_str_mv AT draperwill originsofchemoreceptorcurvaturesortinginescherichiacoli
AT liphardtjan originsofchemoreceptorcurvaturesortinginescherichiacoli