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Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China
To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 bla(CTX-M-55)-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364490/ https://www.ncbi.nlm.nih.gov/pubmed/28338012 http://dx.doi.org/10.1038/srep44866 |
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author | Xia, Liang Liu, Yang Xia, Shu Kudinha, Timothy Xiao, Shu-nian Zhong, Nan-shan Ren, Guo-sheng Zhuo, Chao |
author_facet | Xia, Liang Liu, Yang Xia, Shu Kudinha, Timothy Xiao, Shu-nian Zhong, Nan-shan Ren, Guo-sheng Zhuo, Chao |
author_sort | Xia, Liang |
collection | PubMed |
description | To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 bla(CTX-M-55)-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analysis were performed to characterize plasmids harboring bla(CTX-M-55) and their genetic environment. 111 isolates were clustered into 86 individual pulsotypes and three clusters by PFGE. Fifty-five (49.5%) of the isolates belonged to 8 STs. Most of the ST1193 isolates belonged to one PFGE cluster. Transconjugants (n = 45) derived from randomly selected bla(CTX-M-55) donors (n = 58), were found to contain a single 90-kb conjugative plasmid, which mainly belonged to the IncI1 groups (34, 76%). Among the IncI1 plasmids, the bla(CTX-M-55)/IncI1/ST16 predominated (23/34, 68%). The bla(TEM-1) and aac (3′)-II genes were frequently detected on the IncI1 plasmids, and the insertion of ISEcp1 or IS26 was observed at the 48 bp or 45 bp upstream of the start codon of bla(CTX-M-55) gene. The dissemination of bla(CTX-M-55) gene among E. coli UTI isolates, appeared to be due to both the major clonal lineage of ST1193 and the horizontal transfer of epidemic plasmid IncI1/ST16. |
format | Online Article Text |
id | pubmed-5364490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53644902017-03-28 Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China Xia, Liang Liu, Yang Xia, Shu Kudinha, Timothy Xiao, Shu-nian Zhong, Nan-shan Ren, Guo-sheng Zhuo, Chao Sci Rep Article To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 bla(CTX-M-55)-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analysis were performed to characterize plasmids harboring bla(CTX-M-55) and their genetic environment. 111 isolates were clustered into 86 individual pulsotypes and three clusters by PFGE. Fifty-five (49.5%) of the isolates belonged to 8 STs. Most of the ST1193 isolates belonged to one PFGE cluster. Transconjugants (n = 45) derived from randomly selected bla(CTX-M-55) donors (n = 58), were found to contain a single 90-kb conjugative plasmid, which mainly belonged to the IncI1 groups (34, 76%). Among the IncI1 plasmids, the bla(CTX-M-55)/IncI1/ST16 predominated (23/34, 68%). The bla(TEM-1) and aac (3′)-II genes were frequently detected on the IncI1 plasmids, and the insertion of ISEcp1 or IS26 was observed at the 48 bp or 45 bp upstream of the start codon of bla(CTX-M-55) gene. The dissemination of bla(CTX-M-55) gene among E. coli UTI isolates, appeared to be due to both the major clonal lineage of ST1193 and the horizontal transfer of epidemic plasmid IncI1/ST16. Nature Publishing Group 2017-03-24 /pmc/articles/PMC5364490/ /pubmed/28338012 http://dx.doi.org/10.1038/srep44866 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xia, Liang Liu, Yang Xia, Shu Kudinha, Timothy Xiao, Shu-nian Zhong, Nan-shan Ren, Guo-sheng Zhuo, Chao Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China |
title | Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China |
title_full | Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China |
title_fullStr | Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China |
title_full_unstemmed | Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China |
title_short | Prevalence of ST1193 clone and IncI1/ST16 plasmid in E-coli isolates carrying bla(CTX-M-55) gene from urinary tract infections patients in China |
title_sort | prevalence of st1193 clone and inci1/st16 plasmid in e-coli isolates carrying bla(ctx-m-55) gene from urinary tract infections patients in china |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364490/ https://www.ncbi.nlm.nih.gov/pubmed/28338012 http://dx.doi.org/10.1038/srep44866 |
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