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Halo-free Phase Contrast Microscopy
We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364506/ https://www.ncbi.nlm.nih.gov/pubmed/28338086 http://dx.doi.org/10.1038/srep44034 |
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author | Nguyen, Tan H. Kandel, Mikhail Shakir, Haadi M. Best-Popescu, Catherine Arikkath, Jyothi Do, Minh N. Popescu, Gabriel |
author_facet | Nguyen, Tan H. Kandel, Mikhail Shakir, Haadi M. Best-Popescu, Catherine Arikkath, Jyothi Do, Minh N. Popescu, Gabriel |
author_sort | Nguyen, Tan H. |
collection | PubMed |
description | We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons. |
format | Online Article Text |
id | pubmed-5364506 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53645062017-03-28 Halo-free Phase Contrast Microscopy Nguyen, Tan H. Kandel, Mikhail Shakir, Haadi M. Best-Popescu, Catherine Arikkath, Jyothi Do, Minh N. Popescu, Gabriel Sci Rep Article We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons. Nature Publishing Group 2017-03-24 /pmc/articles/PMC5364506/ /pubmed/28338086 http://dx.doi.org/10.1038/srep44034 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Nguyen, Tan H. Kandel, Mikhail Shakir, Haadi M. Best-Popescu, Catherine Arikkath, Jyothi Do, Minh N. Popescu, Gabriel Halo-free Phase Contrast Microscopy |
title | Halo-free Phase Contrast Microscopy |
title_full | Halo-free Phase Contrast Microscopy |
title_fullStr | Halo-free Phase Contrast Microscopy |
title_full_unstemmed | Halo-free Phase Contrast Microscopy |
title_short | Halo-free Phase Contrast Microscopy |
title_sort | halo-free phase contrast microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364506/ https://www.ncbi.nlm.nih.gov/pubmed/28338086 http://dx.doi.org/10.1038/srep44034 |
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