Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis

BACKGROUND: Lysine-specific histone demethylase 1 (LSD1) modulates chromatin status through demethylation of H3K4 and H3K9. It has been demonstrated that LSD1 is hyperphosphorylated and dissociates from chromatin during mitosis. However, the molecular mechanism of LSD1 detachment is unknown. RESULTS...

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Autores principales: Peng, Bin, Shi, Ruifeng, Jiang, Weiwei, Ding, Yue-He, Dong, Meng-Qiu, Zhu, Wei-Guo, Xu, Xingzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364692/
https://www.ncbi.nlm.nih.gov/pubmed/28344766
http://dx.doi.org/10.1186/s13578-017-0142-x
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author Peng, Bin
Shi, Ruifeng
Jiang, Weiwei
Ding, Yue-He
Dong, Meng-Qiu
Zhu, Wei-Guo
Xu, Xingzhi
author_facet Peng, Bin
Shi, Ruifeng
Jiang, Weiwei
Ding, Yue-He
Dong, Meng-Qiu
Zhu, Wei-Guo
Xu, Xingzhi
author_sort Peng, Bin
collection PubMed
description BACKGROUND: Lysine-specific histone demethylase 1 (LSD1) modulates chromatin status through demethylation of H3K4 and H3K9. It has been demonstrated that LSD1 is hyperphosphorylated and dissociates from chromatin during mitosis. However, the molecular mechanism of LSD1 detachment is unknown. RESULTS: In this report, we found that polo-like kinase 1 (PLK1) directly interacted with LSD1 and phosphorylated LSD1 at Ser-126 . Nocodazole-induced metaphase arrest promoted release of LSD1 from chromatin, and the phosphorylation-defective mutant LSD1 (S126A) failed to dissociate from chromatin upon nocodazole treatment. CONCLUSIONS: Taken together, our findings demonstrate that phosphorylation of LSD1 at Ser-126 by PLK1 promotes its release from chromatin during mitosis.
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spelling pubmed-53646922017-03-24 Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis Peng, Bin Shi, Ruifeng Jiang, Weiwei Ding, Yue-He Dong, Meng-Qiu Zhu, Wei-Guo Xu, Xingzhi Cell Biosci Research BACKGROUND: Lysine-specific histone demethylase 1 (LSD1) modulates chromatin status through demethylation of H3K4 and H3K9. It has been demonstrated that LSD1 is hyperphosphorylated and dissociates from chromatin during mitosis. However, the molecular mechanism of LSD1 detachment is unknown. RESULTS: In this report, we found that polo-like kinase 1 (PLK1) directly interacted with LSD1 and phosphorylated LSD1 at Ser-126 . Nocodazole-induced metaphase arrest promoted release of LSD1 from chromatin, and the phosphorylation-defective mutant LSD1 (S126A) failed to dissociate from chromatin upon nocodazole treatment. CONCLUSIONS: Taken together, our findings demonstrate that phosphorylation of LSD1 at Ser-126 by PLK1 promotes its release from chromatin during mitosis. BioMed Central 2017-03-23 /pmc/articles/PMC5364692/ /pubmed/28344766 http://dx.doi.org/10.1186/s13578-017-0142-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Peng, Bin
Shi, Ruifeng
Jiang, Weiwei
Ding, Yue-He
Dong, Meng-Qiu
Zhu, Wei-Guo
Xu, Xingzhi
Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
title Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
title_full Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
title_fullStr Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
title_full_unstemmed Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
title_short Phosphorylation of LSD1 by PLK1 promotes its chromatin release during mitosis
title_sort phosphorylation of lsd1 by plk1 promotes its chromatin release during mitosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364692/
https://www.ncbi.nlm.nih.gov/pubmed/28344766
http://dx.doi.org/10.1186/s13578-017-0142-x
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