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CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma

Cluster of differentiation 164 (CD164), a sialomucin, has been demonstrated to be involved in the regulation of proliferation, apoptosis, adhesion and differentiation in multiple cancers. CD164 is regarded to be a potential promotor of tumor growth. However, the involvement of CD164 in human glioma...

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Autores principales: Tu, Ming, Cai, Lin, Zheng, Weiming, Su, Zhipeng, Chen, Yong, Qi, Songtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364976/
https://www.ncbi.nlm.nih.gov/pubmed/28259931
http://dx.doi.org/10.3892/mmr.2017.6204
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author Tu, Ming
Cai, Lin
Zheng, Weiming
Su, Zhipeng
Chen, Yong
Qi, Songtao
author_facet Tu, Ming
Cai, Lin
Zheng, Weiming
Su, Zhipeng
Chen, Yong
Qi, Songtao
author_sort Tu, Ming
collection PubMed
description Cluster of differentiation 164 (CD164), a sialomucin, has been demonstrated to be involved in the regulation of proliferation, apoptosis, adhesion and differentiation in multiple cancers. CD164 is regarded to be a potential promotor of tumor growth. However, the involvement of CD164 in human glioma proliferation and apoptosis remains unknown. The aim of the present study was to investigate the expression and oncogenic function of CD164 in normal human astrocytes (NHA) and glioma cells in vitro and in vivo. The results of the present study demonstrated that CD164 mRNA and protein levels were significantly increased in human glioma cell lines and tissue samples. CD164 overexpression promoted the proliferation of NHA in vitro, and its tumorigenic effect was confirmed in a murine xenograft model. Knockdown of CD164 inhibited cell proliferation and promoted apoptosis of the U87 human glioma cell line in vitro and in vivo. In addition, knockdown of CD164 was demonstrated to upregulate the Bax/Bcl2 ratio and phosphatase and tensin homolog (PTEN) expression, reduce protein kinase B (AKT) phosphorylation and promote the expression of p53 in U87 cells. The results suggest that CD164 expression may have affected the proliferation and apoptosis of human glioma cells via the PTEN/phosphoinositide 3-kinase/AKT pathway, and may therefore present a potential target for the diagnosis and treatment of glioma.
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spelling pubmed-53649762017-05-15 CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma Tu, Ming Cai, Lin Zheng, Weiming Su, Zhipeng Chen, Yong Qi, Songtao Mol Med Rep Articles Cluster of differentiation 164 (CD164), a sialomucin, has been demonstrated to be involved in the regulation of proliferation, apoptosis, adhesion and differentiation in multiple cancers. CD164 is regarded to be a potential promotor of tumor growth. However, the involvement of CD164 in human glioma proliferation and apoptosis remains unknown. The aim of the present study was to investigate the expression and oncogenic function of CD164 in normal human astrocytes (NHA) and glioma cells in vitro and in vivo. The results of the present study demonstrated that CD164 mRNA and protein levels were significantly increased in human glioma cell lines and tissue samples. CD164 overexpression promoted the proliferation of NHA in vitro, and its tumorigenic effect was confirmed in a murine xenograft model. Knockdown of CD164 inhibited cell proliferation and promoted apoptosis of the U87 human glioma cell line in vitro and in vivo. In addition, knockdown of CD164 was demonstrated to upregulate the Bax/Bcl2 ratio and phosphatase and tensin homolog (PTEN) expression, reduce protein kinase B (AKT) phosphorylation and promote the expression of p53 in U87 cells. The results suggest that CD164 expression may have affected the proliferation and apoptosis of human glioma cells via the PTEN/phosphoinositide 3-kinase/AKT pathway, and may therefore present a potential target for the diagnosis and treatment of glioma. D.A. Spandidos 2017-04 2017-02-15 /pmc/articles/PMC5364976/ /pubmed/28259931 http://dx.doi.org/10.3892/mmr.2017.6204 Text en Copyright: © Tu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tu, Ming
Cai, Lin
Zheng, Weiming
Su, Zhipeng
Chen, Yong
Qi, Songtao
CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
title CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
title_full CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
title_fullStr CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
title_full_unstemmed CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
title_short CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
title_sort cd164 regulates proliferation and apoptosis by targeting pten in human glioma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364976/
https://www.ncbi.nlm.nih.gov/pubmed/28259931
http://dx.doi.org/10.3892/mmr.2017.6204
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