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Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration

Postharvest physiological deterioration (PPD) is a global challenge in the improvement of cassava value chain. However, how to reduce cassava spoilage and reveal the mechanism of injured cassava storage roots in response to PPD were poorly understood. In the present study, we investigated the activi...

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Autores principales: Qin, Yuling, Djabou, Astride Stéphanie Mouafi, An, Feifei, Li, Kaimian, Li, Zhaogui, Yang, Long, Wang, Xiaojing, Chen, Songbi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365129/
https://www.ncbi.nlm.nih.gov/pubmed/28339481
http://dx.doi.org/10.1371/journal.pone.0174238
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author Qin, Yuling
Djabou, Astride Stéphanie Mouafi
An, Feifei
Li, Kaimian
Li, Zhaogui
Yang, Long
Wang, Xiaojing
Chen, Songbi
author_facet Qin, Yuling
Djabou, Astride Stéphanie Mouafi
An, Feifei
Li, Kaimian
Li, Zhaogui
Yang, Long
Wang, Xiaojing
Chen, Songbi
author_sort Qin, Yuling
collection PubMed
description Postharvest physiological deterioration (PPD) is a global challenge in the improvement of cassava value chain. However, how to reduce cassava spoilage and reveal the mechanism of injured cassava storage roots in response to PPD were poorly understood. In the present study, we investigated the activities of antioxidant enzymes of cassava injured storage roots in PPD-susceptible (SC9) and PPD-tolerant (QZ1) genotypes at the time-points from 0h to 120h, and further analyzed their proteomic changes using two-dimensional electrophoresis (2-DE) in combination with MALDI-TOF-MS/MS. Ninety-nine differentially expressed proteins were identified from SC9 and QZ1 genotypes in the pairwise comparison of 24h/0h, 48h/0h, 72h/0h and 96h/0h. Of those proteins were associated with 13 biological functions, in which carbohydrate and energy metabolism related proteins were the biggest amount differential proteins in both genotypes, followed by chaperones, DNA and RNA metabolism, and defense system. We speculated that SOD in combination with CAT activities would be the first line of defense against PPD to support PPD-tolerant cassava varieties. The four hub proteins including CPN60B, LOS2, HSC70-1 and CPN20B, produced from the network of protein-protein interaction, will be the candidate key proteins linked with PPD. This study provides a new clue to improve cassava PPD-tolerant varieties and would be helpful to much better understand the molecular mechanism of PPD of cassava injured storage roots.
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spelling pubmed-53651292017-04-06 Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration Qin, Yuling Djabou, Astride Stéphanie Mouafi An, Feifei Li, Kaimian Li, Zhaogui Yang, Long Wang, Xiaojing Chen, Songbi PLoS One Research Article Postharvest physiological deterioration (PPD) is a global challenge in the improvement of cassava value chain. However, how to reduce cassava spoilage and reveal the mechanism of injured cassava storage roots in response to PPD were poorly understood. In the present study, we investigated the activities of antioxidant enzymes of cassava injured storage roots in PPD-susceptible (SC9) and PPD-tolerant (QZ1) genotypes at the time-points from 0h to 120h, and further analyzed their proteomic changes using two-dimensional electrophoresis (2-DE) in combination with MALDI-TOF-MS/MS. Ninety-nine differentially expressed proteins were identified from SC9 and QZ1 genotypes in the pairwise comparison of 24h/0h, 48h/0h, 72h/0h and 96h/0h. Of those proteins were associated with 13 biological functions, in which carbohydrate and energy metabolism related proteins were the biggest amount differential proteins in both genotypes, followed by chaperones, DNA and RNA metabolism, and defense system. We speculated that SOD in combination with CAT activities would be the first line of defense against PPD to support PPD-tolerant cassava varieties. The four hub proteins including CPN60B, LOS2, HSC70-1 and CPN20B, produced from the network of protein-protein interaction, will be the candidate key proteins linked with PPD. This study provides a new clue to improve cassava PPD-tolerant varieties and would be helpful to much better understand the molecular mechanism of PPD of cassava injured storage roots. Public Library of Science 2017-03-24 /pmc/articles/PMC5365129/ /pubmed/28339481 http://dx.doi.org/10.1371/journal.pone.0174238 Text en © 2017 Qin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Qin, Yuling
Djabou, Astride Stéphanie Mouafi
An, Feifei
Li, Kaimian
Li, Zhaogui
Yang, Long
Wang, Xiaojing
Chen, Songbi
Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration
title Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration
title_full Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration
title_fullStr Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration
title_full_unstemmed Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration
title_short Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration
title_sort proteomic analysis of injured storage roots in cassava (manihot esculenta crantz) under postharvest physiological deterioration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365129/
https://www.ncbi.nlm.nih.gov/pubmed/28339481
http://dx.doi.org/10.1371/journal.pone.0174238
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