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Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality

OBJECTIVE: Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects. METHODS: This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/...

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Autores principales: de Moura, Bernardo R L, Gurgel, Mariana C A, Machado, Suelen PP, Marques, Priscila A, Rolim, Juliana R, de Lima, Marcelo C, Salgueiro, Lister L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365197/
https://www.ncbi.nlm.nih.gov/pubmed/28333029
http://dx.doi.org/10.5935/1518-0557.20170008
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author de Moura, Bernardo R L
Gurgel, Mariana C A
Machado, Suelen PP
Marques, Priscila A
Rolim, Juliana R
de Lima, Marcelo C
Salgueiro, Lister L
author_facet de Moura, Bernardo R L
Gurgel, Mariana C A
Machado, Suelen PP
Marques, Priscila A
Rolim, Juliana R
de Lima, Marcelo C
Salgueiro, Lister L
author_sort de Moura, Bernardo R L
collection PubMed
description OBJECTIVE: Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects. METHODS: This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal). We analyzed fertilization rate, cleavage, embryo quality at 48 and 72 hours and number of transferred embryos in each group. RESULTS: The diluted enzyme group showed better results in fertilization rates (92.3% vs. 80.6%), mean cleavage (4.18 ± 2.57 vs. 3.09 ± 1.90), in 48-hour embryos A and A + B (60.9% vs. 44.1% and 90.2% vs. 82.3%) and at 72 hours (45.6% vs. 36.8% and 77.1% vs 66.2%), and number of embryos selected for transfer (61.8% vs. 38.1%). The overall pregnancy rate was 59.1%. CONCLUSION: This study demonstrates that the use of 8 IU/mL of hyaluronidase, according to the following protocol, is beneficial and can be successfully used for oocyte denudation, and it is also economically advantageous to the laboratory
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spelling pubmed-53651972017-04-24 Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality de Moura, Bernardo R L Gurgel, Mariana C A Machado, Suelen PP Marques, Priscila A Rolim, Juliana R de Lima, Marcelo C Salgueiro, Lister L JBRA Assist Reprod Original Article OBJECTIVE: Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects. METHODS: This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal). We analyzed fertilization rate, cleavage, embryo quality at 48 and 72 hours and number of transferred embryos in each group. RESULTS: The diluted enzyme group showed better results in fertilization rates (92.3% vs. 80.6%), mean cleavage (4.18 ± 2.57 vs. 3.09 ± 1.90), in 48-hour embryos A and A + B (60.9% vs. 44.1% and 90.2% vs. 82.3%) and at 72 hours (45.6% vs. 36.8% and 77.1% vs 66.2%), and number of embryos selected for transfer (61.8% vs. 38.1%). The overall pregnancy rate was 59.1%. CONCLUSION: This study demonstrates that the use of 8 IU/mL of hyaluronidase, according to the following protocol, is beneficial and can be successfully used for oocyte denudation, and it is also economically advantageous to the laboratory Brazilian Society of Assisted Reproduction 2017 /pmc/articles/PMC5365197/ /pubmed/28333029 http://dx.doi.org/10.5935/1518-0557.20170008 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
de Moura, Bernardo R L
Gurgel, Mariana C A
Machado, Suelen PP
Marques, Priscila A
Rolim, Juliana R
de Lima, Marcelo C
Salgueiro, Lister L
Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
title Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
title_full Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
title_fullStr Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
title_full_unstemmed Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
title_short Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
title_sort low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365197/
https://www.ncbi.nlm.nih.gov/pubmed/28333029
http://dx.doi.org/10.5935/1518-0557.20170008
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