Cargando…
Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus
Diabetes type 2 is associated with impaired insulin production and increased insulin resistance. Treatment with antidiabetic drugs and insulin strives for normalizing glucose homeostasis. In Ethiopian traditional medicine, plant extracts of Melia azedarach are used to control diabetes mellitus and v...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365323/ https://www.ncbi.nlm.nih.gov/pubmed/28360538 http://dx.doi.org/10.2147/JEP.S126146 |
_version_ | 1782517502032478208 |
---|---|
author | Seifu, Daniel Gustafsson, Lars E Chawla, Rajinder Genet, Solomon Debella, Asfaw Holst, Mikael Hellström, Per M |
author_facet | Seifu, Daniel Gustafsson, Lars E Chawla, Rajinder Genet, Solomon Debella, Asfaw Holst, Mikael Hellström, Per M |
author_sort | Seifu, Daniel |
collection | PubMed |
description | Diabetes type 2 is associated with impaired insulin production and increased insulin resistance. Treatment with antidiabetic drugs and insulin strives for normalizing glucose homeostasis. In Ethiopian traditional medicine, plant extracts of Melia azedarach are used to control diabetes mellitus and various gastrointestinal disorders. The objective of this study was to clarify the antidiabetic effects of M. azedarach leaf extracts in diabetic type 2 experimental animals. In this study, mice were injected with Melia extract intraperitoneally. Plasma glucose was studied by using tail vein sampling in acute experiments over 4 h and chronic experiments over 21 days with concurrent insulin and body weight assessments. Glucose tolerance was studied by using intraperitoneal glucose (2 mg/g) tolerance test over 120 min. Gastric emptying of a metabolically inert meal was studied by the gastric retention of a radioactive marker over 20 min. Melia extracts displayed acute, dose-dependent antidiabetic effects in ob/ob mice similar to glibenclamide (p<0.05–0.001). Long-term administration of Melia extract reduced plasma glucose (p<0.001) and insulin (p<0.01–0.001) levels over 21 days, concurrent with body weight loss. Glucose tolerance test showed reduced basal glucose levels (p<0.05–0.01), but no difference was found in glucose disposal after long-term treatment with Melia extract. In addition, the Melia extract at 400 mg/kg slowed gastric emptying rate of normal Sprague-Dawley (p<0.001) and diabetic Goto-Kakizaki rats (p<0.001) compared with controls. It is concluded that the M. azedarach leaf extract elicits diabetic activity through a multitargeted action. Primarily an increased insulin-sensitizing effect is at hand, resulting in blood glucose reduction and improved peripheral glucose disposal, but also through reduced gastric emptying and decreased insulin demand. |
format | Online Article Text |
id | pubmed-5365323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53653232017-03-30 Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus Seifu, Daniel Gustafsson, Lars E Chawla, Rajinder Genet, Solomon Debella, Asfaw Holst, Mikael Hellström, Per M J Exp Pharmacol Original Research Diabetes type 2 is associated with impaired insulin production and increased insulin resistance. Treatment with antidiabetic drugs and insulin strives for normalizing glucose homeostasis. In Ethiopian traditional medicine, plant extracts of Melia azedarach are used to control diabetes mellitus and various gastrointestinal disorders. The objective of this study was to clarify the antidiabetic effects of M. azedarach leaf extracts in diabetic type 2 experimental animals. In this study, mice were injected with Melia extract intraperitoneally. Plasma glucose was studied by using tail vein sampling in acute experiments over 4 h and chronic experiments over 21 days with concurrent insulin and body weight assessments. Glucose tolerance was studied by using intraperitoneal glucose (2 mg/g) tolerance test over 120 min. Gastric emptying of a metabolically inert meal was studied by the gastric retention of a radioactive marker over 20 min. Melia extracts displayed acute, dose-dependent antidiabetic effects in ob/ob mice similar to glibenclamide (p<0.05–0.001). Long-term administration of Melia extract reduced plasma glucose (p<0.001) and insulin (p<0.01–0.001) levels over 21 days, concurrent with body weight loss. Glucose tolerance test showed reduced basal glucose levels (p<0.05–0.01), but no difference was found in glucose disposal after long-term treatment with Melia extract. In addition, the Melia extract at 400 mg/kg slowed gastric emptying rate of normal Sprague-Dawley (p<0.001) and diabetic Goto-Kakizaki rats (p<0.001) compared with controls. It is concluded that the M. azedarach leaf extract elicits diabetic activity through a multitargeted action. Primarily an increased insulin-sensitizing effect is at hand, resulting in blood glucose reduction and improved peripheral glucose disposal, but also through reduced gastric emptying and decreased insulin demand. Dove Medical Press 2017-03-20 /pmc/articles/PMC5365323/ /pubmed/28360538 http://dx.doi.org/10.2147/JEP.S126146 Text en © 2017 Seifu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Seifu, Daniel Gustafsson, Lars E Chawla, Rajinder Genet, Solomon Debella, Asfaw Holst, Mikael Hellström, Per M Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
title | Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
title_full | Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
title_fullStr | Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
title_full_unstemmed | Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
title_short | Antidiabetic and gastric emptying inhibitory effect of herbal Melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
title_sort | antidiabetic and gastric emptying inhibitory effect of herbal melia azedarach leaf extract in rodent models of diabetes type 2 mellitus |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5365323/ https://www.ncbi.nlm.nih.gov/pubmed/28360538 http://dx.doi.org/10.2147/JEP.S126146 |
work_keys_str_mv | AT seifudaniel antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus AT gustafssonlarse antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus AT chawlarajinder antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus AT genetsolomon antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus AT debellaasfaw antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus AT holstmikael antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus AT hellstromperm antidiabeticandgastricemptyinginhibitoryeffectofherbalmeliaazedarachleafextractinrodentmodelsofdiabetestype2mellitus |